HXK I Antibody (G-1): sc-46695

HXK I Antibody (G-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects HXK I of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). HXK I (G-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HXK I plays a crucial role in glucose metabolism, catalyzing the conversion of glucose to glucose-6-phosphate, which is the first step in the glycolytic pathway. This enzymatic activity is vital for cellular energy production and metabolic regulation, particularly in tissues with high energy demands such as the brain, heart, and kidneys, where HXK I is predominantly expressed. The regulation of glucose metabolism by HXK I is essential for maintaining glucose homeostasis, and dysfunction can lead to metabolic disorders, including diabetes. Additionally, HXK I is one of four hexokinase isoenzymes, each with distinct regulatory properties and tissue distributions, highlighting the importance of HXK I in the broader context of glucose metabolism and its implications for health and disease.

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HXK I Antibody (G-1) References:

1. Human glucokinase gene: isolation, characterization, and identification of two missense mutations linked to early-onset non-insulin-dependent (type 2) diabetes mellitus.  |  Stoffel, M., et al. 1992. Proc Natl Acad Sci U S A. 89: 7698-702. PMID: 1502186
2. Glucose phosphorylation in tumor cells. Cloning, sequencing, and overexpression in active form of a full-length cDNA encoding a mitochondrial bindable form of hexokinase.  |  Arora, KK., et al. 1990. J Biol Chem. 265: 6481-8. PMID: 2318862
3. A Tumor Agnostic Therapeutic Strategy for Hexokinase 1-Null/Hexokinase 2-Positive Cancers.  |  Xu, S. and Herschman, HR. 2019. Cancer Res. 79: 5907-5914. PMID: 31434645
4. Hexokinase 1 cellular localization regulates the metabolic fate of glucose.  |  De Jesus, A., et al. 2022. Mol Cell. 82: 1261-1277.e9. PMID: 35305311
5. HK1 from hepatic stellate cell-derived extracellular vesicles promotes progression of hepatocellular carcinoma.  |  Chen, QT., et al. 2022. Nat Metab. 4: 1306-1321. PMID: 36192599
6. A de novo hexokinase 1 (HK1) variant presenting as Boucher-Neuhäuser syndrome.  |  Peretz, RH., et al. 2023. Am J Med Genet A. 191: 624-629. PMID: 36541585
7. Targeting hexokinase 1 alleviates NLRP3-mediated inflammation in apical periodontitis: A laboratory investigation.  |  Qian, Y., et al. 2023. Int Endod J. 56: 734-747. PMID: 36938709
8. Biallelic hexokinase 1 (HK1) variants causative of non-spherocytic haemolytic anaemia: A case series with emphasis on the HK1 promoter variant and literature review.  |  Ukonmaanaho, EM., et al. 2024. Br J Haematol. 204: 2040-2048. PMID: 38415930
9. Hexokinase 1 forms rings that regulate mitochondrial fission during energy stress.  |  Pilic, J., et al. 2024. Mol Cell. 84: 2732-2746.e5. PMID: 38981483
10. Multiple forms of hexokinase in the rat: tissue distribution, age dependency, and properties.  |  Katzen, HM. and Schimke, RT. 1965. Proc Natl Acad Sci U S A. 54: 1218-25. PMID: 5219826
11. Human hexokinase II: sequence and homology to other hexokinases.  |  Deeb, SS., et al. 1993. Biochem Biophys Res Commun. 197: 68-74. PMID: 8250948
12. Purification and characterization of the carboxyl-domain of human hexokinase type III expressed as fusion protein.  |  Palma, F., et al. 1996. Mol Cell Biochem. 155: 23-9. PMID: 8717435

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HXK I Antibody (G-1) has 27 citations in a variety of scientific publications.

Primary antibodies like Anti-HXK I Antibody (G-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HXK I Antibody (G-1) Specifications

Alternate Product Name: Hexokinase I

Product Clone Name: G-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 316-410

Epitope Target: HXK I

Epitope Species: human

Product Reactivity/Detection: HXK I

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 27 citations in various scientific publications

Available Conjugates/Forms for HXK I Antibody (G-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin