Hugl-1 Antibody (A-2): sc-136992
- Hugl-1 Antibody (A-2) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 937-1062 mapping at the C-terminus of Hugl-1 of human origin
- recommended for detection of Hugl-1 of human origin by WB, IP, IF and ELISA
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Hugl-1 Antibody (A-2) for WB applications. These reagents are now offered in bundles with Hugl-1 Antibody (A-2) (see ordering information below).
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SEE ALSO...
Hugl-1 Antibody (A-2) is a mouse monoclonal IgG1 antibody that detects Hugl-1 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Hugl-1 is a crucial cortical cytoskeleton protein that plays a significant role in regulating mitotic spindle orientation, differentiation, proliferation, and organization of neuroepithelial cells. Hugl-1 is primarily localized in the cytoplasm, where Hugl-1 participates in a complex essential for maintaining cell polarity and epithelial integrity. Hugl-1 interacts with several key partners, including nonmuscle Myosin II heavy chain, PRKCI/aPKC, PARD6B/Par-6, PARD6A, and STX4A, which are vital for cellular functions such as adhesion and migration. Notably, Hugl-1 functions as a tumor suppressor in humans, and Hugl-1 expression is critical for preventing progression of colorectal cancer and melanoma. The gene encoding Hugl-1, LLGL1, shares significant homology with the Drosophila tumor suppressor gene l(2)gl, which encodes Lgl protein, underscoring evolutionary conservation of its function in maintaining cellular architecture. Hugl-1 expression is observed in various tissues, including kidney, brain, and muscle, where Hugl-1 enhances cell adhesion and reduces cell migration, highlighting importance in tissue organization and integrity.
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Hugl-1 Antibody (A-2) References:
- Expression of Hugl-1 is strongly reduced in malignant melanoma. | Kuphal, S., et al. 2006. Oncogene. 25: 103-10. PMID: 16170365
- Loss of Hugl-1 expression associates with lymph node metastasis in endometrial cancer. | Tsuruga, T., et al. 2007. Oncol Res. 16: 431-5. PMID: 18074678
- Regulation of the hDlg/hScrib/Hugl-1 tumour suppressor complex. | Massimi, P., et al. 2008. Exp Cell Res. 314: 3306-17. PMID: 18793635
- Aberrant splicing of Hugl-1 is associated with hepatocellular carcinoma progression. | Lu, X., et al. 2009. Clin Cancer Res. 15: 3287-96. PMID: 19447873
- Preservation of HUGL-1 expression as a favourable prognostic factor in pancreatic carcinoma. | Biesterfeld, S., et al. 2012. Anticancer Res. 32: 3153-9. PMID: 22843887
- Hugl1 and Hugl2 in mammary epithelial cells: polarity, proliferation, and differentiation. | Russ, A., et al. 2012. PLoS One. 7: e47734. PMID: 23110097
- Hugl-1 induces apoptosis in esophageal carcinoma cells both in vitro and in vivo. | Song, J., et al. 2013. World J Gastroenterol. 19: 4127-36. PMID: 23864775
- Hugl-1 inhibits glioma cell growth in intracranial model. | Liu, X., et al. 2015. J Neurooncol. 125: 113-21. PMID: 26341367
- Reduced Expression of Hugl 1 Contributes to the Progression of Lung Squamous Cell Carcinoma. | Matsuzaki, T., et al. 2015. Tokai J Exp Clin Med. 40: 169-77. PMID: 26662669
- Human giant larvae-1 promotes migration and invasion of malignant glioma cells by regulating N-cadherin. | Wang, Y., et al. 2021. Oncol Lett. 21: 167. PMID: 33552285
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hugl-1 Antibody (A-2) | sc-136992 | 200 µg/ml | $316.00 | |||
Hugl-1 Antibody (A-2): m-IgG Fc BP-HRP Bundle | sc-539914 | 200 µg Ab; 10 µg BP | $354.00 | |||
Hugl-1 Antibody (A-2): m-IgG1 BP-HRP Bundle | sc-541782 | 200 µg Ab; 20 µg BP | $354.00 |