Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HtrA2 Double Nickase Plasmid (h): sc-402576-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HtrA2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HtrA2 Double Nickase Plasmid (h) and HtrA2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HTRA2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HtrA2 Antibody (E-3): sc-365594
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HtrA2 Double Nickase Plasmid (h) | sc-402576-NIC | 20 µg | $410.00 |
HTRA2 encodes HtrA2 (also known as Omi), a mitochondrial serine protease that contributes to protein quality control and mitochondrial homeostasis. Following cellular stress, HtrA2 can be released into the cytosol where it modulates apoptosis by antagonizing inhibitor of apoptosis proteins (IAPs) and influencing caspase activation. The protein is also linked to mitochondrial dynamics, unfolded protein stress responses, and proteostasis pathways that shape cellular survival decisions. Dysregulated HTRA2 activity or expression has been associated with neurodegeneration and other conditions characterized by mitochondrial dysfunction and altered apoptotic signaling.
HtrA2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HTRA2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HTRA2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HTRA2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HTRA2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.