HSV-2 gB Antibody (1-G-10): sc-52425

HSV-2 gB Antibody (1-G-10) is a mouse monoclonal IgG1 antibody that detects HSV-2 gB of Herpes Simplex Virus 2 origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-HSV-2 gB antibody (1-G-10) is available in a non-conjugated form, providing researchers with a versatile tool for studying this critical viral protein. Glycoprotein B (gB) plays a vital role in the viral life cycle, particularly in the process of membrane fusion, which is essential for the entry and spread of enveloped viruses like herpes simplex virus. HSV-2 gB is crucial for viral growth, as virions lacking gB can bind to host cells but fail to initiate the synthesis of virus-specific proteins upon infection. The structure of gB is particularly important; gB exists as a homodimer linked by disulfide bonds, with both HSV-1 and HSV-2 gB proteins comprising 904 amino acids. The extracellular domain of HSV-2 gB spans amino acids 23-727, while the cytoplasmic domain includes amino acids 793-904. Understanding the structure and function of gB is essential for developing therapeutic strategies against herpes simplex virus infections, as gB is a key target for neutralizing antibodies and antiviral drugs.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

HSV-2 gB Antibody (1-G-10) References:

1. Characterization of cell-cell fusion mediated by herpes simplex virus 2 glycoproteins gB, gD, gH and gL in transfected cells.  |  Muggeridge, MI. 2000. J Gen Virol. 81: 2017-2027. PMID: 10900041
2. Assembly and organization of glycoproteins B, C, D, and H in herpes simplex virus type 1 particles lacking individual glycoproteins: No evidence for the formation of a complex of these molecules.  |  Rodger, G., et al. 2001. J Virol. 75: 710-6. PMID: 11134284
3. Plasma membrane requirements for cell fusion induced by herpes simplex virus type 1 glycoproteins gB, gD, gH and gL.  |  Browne, H., et al. 2001. J Gen Virol. 82: 1419-1422. PMID: 11369886
4. Truncation of herpes simplex virus type 2 glycoprotein B increases its cell surface expression and activity in cell-cell fusion, but these properties are unrelated.  |  Fan, Z., et al. 2002. J Virol. 76: 9271-83. PMID: 12186911
5. Topical herpes simplex virus 2 (HSV-2) vaccination with human papillomavirus vectors expressing gB/gD ectodomains induces genital-tissue-resident memory CD8+ T cells and reduces genital disease and viral shedding after HSV-2 challenge.  |  Çuburu, N., et al. 2015. J Virol. 89: 83-96. PMID: 25320297
6. Contribution of N-linked glycans on HSV-2 gB to cell-cell fusion and viral entry.  |  Luo, S., et al. 2015. Virology. 483: 72-82. PMID: 25965797
7. Patient-Specific Neutralizing Antibody Responses to Herpes Simplex Virus Are Attributed to Epitopes on gD, gB, or Both and Can Be Type Specific.  |  Cairns, TM., et al. 2015. J Virol. 89: 9213-31. PMID: 26109729
8. Role of glycoprotein B of herpes simplex virus type 1 in viral entry and cell fusion.  |  Cai, WH., et al. 1988. J Virol. 62: 2596-604. PMID: 2839688
9. Seroepidemiology and control of genital herpes: the value of type specific antibodies to herpes simplex virus.  |  Slomka, MJ. 1996. Commun Dis Rep CDR Rev. 6: R41-5. PMID: 8820192
10. Disulfide bonds of herpes simplex virus type 2 glycoprotein gB.  |  Norais, N., et al. 1996. J Virol. 70: 7379-87. PMID: 8892856
11. Genetic immunization with adeno-associated virus vectors expressing herpes simplex virus type 2 glycoproteins B and D.  |  Manning, WC., et al. 1997. J Virol. 71: 7960-2. PMID: 9311887
12. Glycoproteins gB, gD, and gHgL of herpes simplex virus type 1 are necessary and sufficient to mediate membrane fusion in a Cos cell transfection system.  |  Turner, A., et al. 1998. J Virol. 72: 873-5. PMID: 9420303

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HSV-2 gB Antibody (1-G-10) has 3 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HSV-2 gB Antibody (1-G-10) Specifications

Alternate Product Name: HSV-2 gB

Product Clone Name: 1-G-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: HSV-2 gB

Epitope Species: HSV

Product Reactivity/Detection: HSV-2 gB

Species Reactivity/Detection: Herpes Simplex Virus 2

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 100 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for HSV-2 gB Antibody (1-G-10): N/A

The preferred HSV-1/2 gB antibody is HSV-1/2 gB Antibody (10B7): sc-56987.