HSV-1/2 ICP35 Antibody (H745): sc-69808

HSV-1/2 ICP35 Antibody (H745) is a mouse monoclonal IgG3 antibody that detects HSV-1/2 ICP35 of HSV-1 and HSV-2 origin by immunofluorescence (IF). Anti-HSV-1/2 ICP35 antibody (H745) is available as the non-conjugated format. Infected Cell Polypeptide 35 (ICP35) plays a crucial role in the herpes simplex virus life cycle, functioning as a scaffolding protein integral to viral nucleocapsid assembly. ICP35 is essential for forming sealed viral capsids, necessary for protecting viral genetic material and successful virus propagation. ICP35 interacts with itself and with major capsid protein VP5, facilitating viral capsid structural integrity and stability. Understanding ICP35 function is vital, as ICP35 not only contributes to viral replication but also represents a potential target for therapeutic interventions aimed at disrupting herpes simplex virus lifecycle.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

HSV-1/2 ICP35 Antibody (H745) References:

1. Co-localization of HSV-1 DNA and ICP35 protein by in situ hybridization and immunocytochemistry.  |  Morioka, H., et al. 1999. J Electron Microsc (Tokyo). 48: 621-8. PMID: 10605095
2. Herpes simplex virus UL17 protein is associated with B capsids and colocalizes with ICP35 and VP5 in infected cells.  |  Goshima, F., et al. 2000. Arch Virol. 145: 417-26. PMID: 10752563
3. Processing of the herpes simplex virus assembly protein ICP35 near its carboxy terminal end requires the product of the whole of the UL26 reading frame.  |  Preston, VG., et al. 1992. Virology. 186: 87-98. PMID: 1309284
4. Herpes simplex virus type 1 protease expressed in Escherichia coli exhibits autoprocessing and specific cleavage of the ICP35 assembly protein.  |  Deckman, IC., et al. 1992. J Virol. 66: 7362-7. PMID: 1331526
5. Monoclonal antibodies against herpes simplex virus type 1-infected nuclei defining and localizing the ICP8 protein, 65K DNA-binding protein and polypeptides of the ICP35 family.  |  Schenk, P., et al. 1988. J Gen Virol. 69 (Pt 1): 99-111. PMID: 2826672
6. Antibody to herpes simplex virus (HSV) ICP-35 proteins after HSV challenge of animals immunized with HSV subunit vaccines.  |  Bernstein, DI., et al. 1988. J Infect Dis. 157: 1178-86. PMID: 2836519
7. The C-terminal 25 amino acids of the protease and its substrate ICP35 of herpes simplex virus type 1 are involved in the formation of sealed capsids.  |  Matusick-Kumar, L., et al. 1995. J Virol. 69: 4347-56. PMID: 7769696
8. Phenotype of the herpes simplex virus type 1 protease substrate ICP35 mutant virus.  |  Matusick-Kumar, L., et al. 1994. J Virol. 68: 5384-94. PMID: 8057422
9. Separate functional domains of the herpes simplex virus type 1 protease: evidence for cleavage inside capsids.  |  Robertson, BJ., et al. 1996. J Virol. 70: 4317-28. PMID: 8676454
10. Na, an autoproteolytic product of the herpes simplex virus type 1 protease, can functionally substitute for the assembly protein ICP35.  |  Robertson, BJ., et al. 1997. J Virol. 71: 1683-7. PMID: 8995700
11. Nucleotide sequence of the herpes simplex virus type 2 gene encoding the protease and capsid protein ICP35.  |  Steffy, KR., et al. 1995. J Gen Virol. 76 (Pt 4): 1069-72. PMID: 9049360
12. Self-association of herpes simplex virus type 1 ICP35 is via coiled-coil interactions and promotes stable interaction with the major capsid protein.  |  Pelletier, A., et al. 1997. J Virol. 71: 5197-208. PMID: 9188587

The preferred HSV-1 antibody is HSV-1 Antibody (20.7.1): sc-57863.