HSPC144 CRISPR/Cas9 KO Plasmid (h): sc-412026
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HSPC144 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HSPC144 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HSPC144 CRISPR/Cas9 KO Plasmid (h) | sc-412026 | 20 µg | $397.00 |
Overview
THYN1 (also known as HSPC144) encodes a thymocyte nuclear protein implicated in regulation of apoptosis and cell-cycle control, with reported roles in maintaining cellular homeostasis during proliferation and stress. The protein has been linked to nuclear processes that influence survival signaling and may intersect with pathways governing programmed cell death, including caspase-dependent mechanisms. Altered THYN1 expression has been observed in multiple tumor contexts, suggesting relevance to oncogenic transformation and tumor cell fitness. As a result, THYN1 is frequently studied in models of hematopoietic and solid-tissue biology to clarify how apoptosis regulation contributes to disease-associated phenotypes.
HSPC144 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the THYN1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the THYN1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the THYN1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HSPC144 protein expression.
This CRISPR knockout system enables efficient generation of THYN1-deficient cell models for investigation of HSPC144 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting THYN1 exon(s) critical for HSPC144 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple THYN1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HSPC144 CRISPR/Cas9 KO Plasmid (h) and HSPC144 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the THYN1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HSPC144 HDR Plasmid (h) and HSPC144 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by THYN1 homology arms to support homology-directed repair at defined THYN1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.