HSPA6 Double Nickase Plasmid (h): sc-400870-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HSPA6 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HSPA6 Double Nickase Plasmid (h) and HSPA6 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HSPA6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HSPA6 Antibody (G-9): sc-374589
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HSPA6 Double Nickase Plasmid (h) | sc-400870-NIC | 20 µg | $410.00 | |||
HSPA6 Double Nickase Plasmid (h2) | sc-400870-NIC-2 | 20 µg | $410.00 |
HSPA6 encodes a stress-inducible member of the HSP70 chaperone family that stabilizes nascent and damaged proteins and helps restore proteostasis following acute cellular stress. HSPA6 participates in ATP-dependent protein folding, prevention of protein aggregation, and recovery of the proteome after heat shock, oxidative stress, and proteotoxic insults, interfacing with pathways such as the heat shock response, unfolded protein handling, and protein quality control. By influencing folding capacity and chaperone availability, HSPA6 expression can modulate cellular susceptibility to stress-induced dysfunction, making it relevant for studies of proteostasis imbalance observed across neurodegeneration, cancer cell stress adaptation, and inflammatory microenvironments.
HSPA6 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HSPA6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HSPA6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HSPA6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HSPA6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.