HSPA14 Double Nickase Plasmid (h): sc-407280-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HSPA14 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HSPA14 Double Nickase Plasmid (h) and HSPA14 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HSPA14. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HSPA14 Antibody (G-9): sc-398208
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HSPA14 Double Nickase Plasmid (h) | sc-407280-NIC | 20 µg | $410.00 |
HSPA14 encodes a member of the HSP70 family that functions as an ATP-dependent molecular chaperone supporting nascent polypeptide folding, protein quality control, and proteostasis maintenance under basal and stress conditions. HSPA14 activity intersects with heat-shock responses and co-chaperone networks to regulate translation-associated folding and limit accumulation of misfolded proteins. Through these roles, it influences cellular stress resilience, proteotoxic stress signaling, and recovery from environmental or metabolic insults that perturb protein homeostasis. Dysregulation of chaperone capacity, including HSP70-family components such as HSPA14, is frequently studied in contexts of neurodegeneration, cancer cell stress adaptation, and disorders marked by impaired proteome maintenance.
HSPA14 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HSPA14 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HSPA14. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HSPA14 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HSPA14-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.