Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HSP 60 CRISPR/Cas9 KO Plasmid (h): sc-400336
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HSP 60 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HSP 60 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HSP 60 Antibody (LK1): sc-59567
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HSP 60 CRISPR/Cas9 KO Plasmid (h) | sc-400336 | 20 µg | $397.00 |
Overview
HSPD1 encodes the mitochondrial chaperonin HSP 60, a central component of the chaperonin system that promotes folding and assembly of nuclear-encoded proteins imported into the mitochondrial matrix. HSP 60 supports mitochondrial proteostasis, oxidative phosphorylation capacity, and stress responses by buffering protein misfolding and limiting proteotoxic damage. Perturbation of HSPD1 disrupts mitochondrial quality control and can alter apoptosis susceptibility, redox homeostasis, and innate immune signaling linked to mitochondrial dysfunction. Dysregulated HSP 60 expression or localization has been associated with neurodegeneration, cardiometabolic disorders, and cancer-related stress adaptation, making it a valuable node for mechanistic studies of mitochondrial signaling.
HSP 60 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HSPD1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HSPD1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HSPD1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HSP 60 protein expression.
This CRISPR knockout system enables efficient generation of HSPD1-deficient cell models for investigation of HSP 60 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HSPD1 exon(s) critical for HSP 60 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HSPD1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HSP 60 CRISPR/Cas9 KO Plasmid (h) and HSP 60 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HSPD1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HSP 60 HDR Plasmid (h) and HSP 60 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HSPD1 homology arms to support homology-directed repair at defined HSPD1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.