HSP 40-4 Double Nickase Plasmid (h): sc-403819-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HSP 40-4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HSP 40-4 Double Nickase Plasmid (h) and HSP 40-4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DNAJA1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HSP 40-4 Antibody (KA2A5.6): sc-59554
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HSP 40-4 Double Nickase Plasmid (h) | sc-403819-NIC | 20 µg | $410.00 | |||
HSP 40-4 Double Nickase Plasmid (h2) | sc-403819-NIC-2 | 20 µg | $410.00 |
DNAJA1 encodes the human HSP40 family co-chaperone HSP 40-4 (also known as Hdj2), a J-domain protein that stimulates HSP70 ATPase activity to support protein folding, refolding, and quality control. Through interactions with HSP70 and client proteins, DNAJA1 contributes to proteostasis across the cytosol and at organelle-associated interfaces, influencing stress responses, protein trafficking, and degradation pathways such as the ubiquitin–proteasome system. Dysregulation of chaperone networks involving DNAJA1 has been linked to altered cellular tolerance to proteotoxic stress, with relevance to cancer biology and neurodegeneration where misfolded protein burden and stress signaling are prominent. DNAJA1 function is also studied in the context of host–pathogen interactions and signaling pathway stability, reflecting its role in maintaining functional protein complexes.
HSP 40-4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DNAJA1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DNAJA1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DNAJA1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DNAJA1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.