The Double Nickase Plasmid features a U6 promoter for sgRNA expression, a 20 nt targeting sequence, and a gRNA scaffold to guide Cas9n. It includes a CBh promoter for Cas9n (D10A) and puromycin resistance, GFP for transfection verification, and nuclear localization signals (NLS). The 2A peptide allows co-expression of Cas9n and Puro from a single promoter, enabling precise genome editing with reduced off-target effects.
HSP 27 Double Nickase Plasmid (h): sc-400285-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HSP 27 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HSP 27 Double Nickase Plasmid (h) and HSP 27 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HSPB1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HSP 27 Antibody (F-4): sc-13132
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HSP 27 Double Nickase Plasmid (h) | sc-400285-NIC | 20 µg | $410.00 | |||
HSP 27 Double Nickase Plasmid (h2) | sc-400285-NIC-2 | 20 µg | $410.00 |