HS6ST2 CRISPR/Cas9 KO Plasmid (h): sc-407513
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HS6ST2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HS6ST2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HS6ST2 CRISPR/Cas9 KO Plasmid (h) | sc-407513 | 20 µg | $397.00 |
Overview
HS6ST2 encodes heparan sulfate 6-O-sulfotransferase 2, a Golgi-resident enzyme that catalyzes 6-O-sulfation of glucosamine residues during heparan sulfate proteoglycan biosynthesis. This modification shapes heparan sulfate sulfation patterns that regulate ligand binding and co-receptor functions, influencing signaling pathways such as FGF, VEGF, WNT, BMP, and chemokine-mediated cues. By tuning extracellular matrix and cell-surface glycosaminoglycan interactions, HS6ST2 contributes to cell adhesion, migration, and developmental patterning. Altered heparan sulfate sulfation has been associated with dysregulated growth factor signaling and tumor microenvironment remodeling, supporting investigation of HS6ST2 in cancer biology and other disorders linked to extracellular matrix–dependent signaling.
HS6ST2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HS6ST2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HS6ST2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HS6ST2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HS6ST2 protein expression.
This CRISPR knockout system enables efficient generation of HS6ST2-deficient cell models for investigation of HS6ST2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HS6ST2 exon(s) critical for HS6ST2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HS6ST2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HS6ST2 CRISPR/Cas9 KO Plasmid (h) and HS6ST2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HS6ST2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HS6ST2 HDR Plasmid (h) and HS6ST2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HS6ST2 homology arms to support homology-directed repair at defined HS6ST2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.