HS3ST3B1 CRISPR/Cas9 KO Plasmid (m): sc-424928
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HS3ST3B1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HS3ST3B1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HS3ST3B1 CRISPR/Cas9 KO Plasmid (m) | sc-424928 | 20 µg | $397.00 |
Overview
Hs3st3b1 encodes heparan sulfate-glucosamine 3-O-sulfotransferase 3B1 (HS3ST3B1), a Golgi-localized enzyme that installs 3-O-sulfate groups on heparan sulfate chains. This modification tunes heparan sulfate proteoglycan binding to growth factors, chemokines, and extracellular matrix components, shaping signaling and adhesion processes such as FGF and VEGF pathway modulation and cell–cell communication. Altered heparan sulfate sulfation patterns have been linked to dysregulated morphogen gradients, inflammatory signaling, and tumor–stromal interactions, making HS3ST3B1 relevant to studies of microenvironmental regulation and receptor–ligand specificity. In mouse systems, Hs3st3b1 perturbation supports mechanistic interrogation of glycosaminoglycan structure–function relationships in development and tissue homeostasis.
HS3ST3B1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Hs3st3b1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Hs3st3b1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Hs3st3b1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HS3ST3B1 protein expression.
This CRISPR knockout system enables efficient generation of Hs3st3b1-deficient cell models for investigation of HS3ST3B1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Hs3st3b1 exon(s) critical for HS3ST3B1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Hs3st3b1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HS3ST3B1 CRISPR/Cas9 KO Plasmid (m) and HS3ST3B1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Hs3st3b1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HS3ST3B1 HDR Plasmid (m) and HS3ST3B1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Hs3st3b1 homology arms to support homology-directed repair at defined Hs3st3b1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.