Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HS3ST2 CRISPR/Cas9 KO Plasmid (h): sc-409766
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HS3ST2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HS3ST2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HS3ST2 Antibody (G-6): sc-514361
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HS3ST2 CRISPR/Cas9 KO Plasmid (h) | sc-409766 | 20 µg | $397.00 |
Overview
HS3ST2 encodes heparan sulfate-glucosamine 3-O-sulfotransferase 2, a Golgi-localized enzyme that catalyzes 3-O-sulfation of heparan sulfate chains, generating rare sulfation motifs that modulate ligand binding and signaling specificity. By shaping heparan sulfate–protein interactions, HS3ST2 influences extracellular matrix biology and regulates pathways such as FGF, WNT, VEGF, and chemokine-driven signaling that control cell adhesion, migration, and tissue patterning. Altered HS3ST2 expression or epigenetic silencing has been reported in multiple tumor contexts and is studied in relation to invasion, metastasis-associated microenvironment remodeling, and dysregulated growth factor responsiveness. Its role in heparan sulfate biosynthesis also links it to broader questions in developmental biology and inflammation where sulfation patterns tune receptor activation and gradient formation.
HS3ST2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HS3ST2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HS3ST2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HS3ST2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HS3ST2 protein expression.
This CRISPR knockout system enables efficient generation of HS3ST2-deficient cell models for investigation of HS3ST2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HS3ST2 exon(s) critical for HS3ST2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HS3ST2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HS3ST2 CRISPR/Cas9 KO Plasmid (h) and HS3ST2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HS3ST2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HS3ST2 HDR Plasmid (h) and HS3ST2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HS3ST2 homology arms to support homology-directed repair at defined HS3ST2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.