Hrs Antibody (D-6): sc-166843

Hrs Antibody (D-6) is a mouse monoclonal IgG2a antibody that detects hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Hrs antibody (D-6) recognizes Hrs, a crucial zinc-finger protein that plays a significant role in intracellular trafficking and signal transduction, particularly associated with early endosomes. Hrs (D-6) monoclonal antibody targets a unique structure including a phosphatidylinositol 3-phosphate-binding domain, which is vital for endosomal targeting, allowing Hrs to co-localize with Clathrin at the carboxy-terminus through a Clathrin box motif. This interaction is essential for proper functioning of endosomal sorting processes, which are critical for maintaining cellular homeostasis and regulating signaling pathways. Additionally, Hrs is involved in ventral folding morphogenesis and shares structural similarities with the yeast protein Vps27p, known for its role in vacuolar protein sorting. The human Hrs gene, located on chromosome 17q25, encodes a 777 amino acid protein, and in Schwann cells, Hrs co-localizes with the tumor suppressor protein schwannomin at endosomes, indicating a potential collaborative role in Hrs-mediated cell signaling.

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Hrs Antibody (D-6) References:

1. Hepatocyte growth factor-regulated tyrosine kinase substrate (HRS) interacts with PELP1 and activates MAPK.  |  Rayala, SK., et al. 2006. J Biol Chem. 281: 4395-403. PMID: 16352611
2. Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS) and guanylate kinase 1 (GUK1) are differentially expressed in GH-secreting adenomas.  |  da Rocha, AA., et al. 2006. Pituitary. 9: 83-92. PMID: 16832584
3. Hepatocyte growth factor-regulated tyrosine kinase substrate (HRS) mediates post-endocytic trafficking of protease-activated receptor 2 and calcitonin receptor-like receptor.  |  Hasdemir, B., et al. 2007. J Biol Chem. 282: 29646-57. PMID: 17675298
4. Hepatocyte growth factor-regulated tyrosine kinase substrate (Hgs) is involved in BMP signaling through phosphorylation of SMADS and TAK1 in early mouse embryo.  |  Miura, S. and Mishina, Y. 2011. Dev Dyn. 240: 2474-81. PMID: 21953618
5. Hepatocyte growth factor regulated tyrosine kinase substrate in the peripheral development and function of B-cells.  |  Nagata, T., et al. 2014. Biochem Biophys Res Commun. 443: 351-6. PMID: 24246674
6. ESCRT-0 protein hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) is targeted to endosomes independently of signal-transducing adaptor molecule (STAM) and the complex formation with STAM promotes its endosomal dissociation.  |  Kojima, K., et al. 2014. J Biol Chem. 289: 33296-310. PMID: 25296754
7. ESCRT-0 protein hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) is targeted to endosomes independently of signal-transducing adaptor molecule (STAM) and the complex formation with STAM promotes its endosomal dissociation.  |  Kojima, K., et al. 2015. J Biol Chem. 290: 8065-6. PMID: 25819836
8. The Role of Ubiquitination and Hepatocyte Growth Factor-Regulated Tyrosine Kinase Substrate in the Degradation of the Adrenomedullin Type I Receptor.  |  Roux, BT., et al. 2017. Sci Rep. 7: 12389. PMID: 28959041
9. Hepatocyte growth factor-regulated tyrosine kinase substrate is essential for endothelial cell polarity and cerebrovascular stability.  |  Yu, Z., et al. 2021. Cardiovasc Res. 117: 533-546. PMID: 32044971
10. HRS Regulates Small Extracellular Vesicle PD-L1 Secretion and Is Associated with Anti-PD-1 Treatment Efficacy.  |  Xiao, BL., et al. 2023. Cancer Immunol Res. 11: 228-240. PMID: 36484721

The preferred Hrs antibody is Hrs Antibody (C-7): sc-271455.