HPV18 E7 Antibody (F-7): sc-365035

HPV18 E7 Antibody (F-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects HPV18 E7 protein by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HPV18 E7 Antibody (F-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HPV18 E7 protein plays a critical role in cervical cancer pathogenesis by interacting with retinoblastoma protein (pRB), leading to cell cycle regulation disruption. This interaction is significant because pRB is a key tumor suppressor that normally inhibits cell cycle progression; thus, E7 binding to pRB results in E2F transcription factor release, promoting uncontrolled cell division and contributing to oncogenesis. High-risk HPV types, including HPV18, are found in approximately 90% of cervical cancer cases, making HPV18 E7 protein detection and study vital for understanding HPV-related malignancies and developing targeted therapies.

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HPV18 E7 Antibody (F-7) References:

1. Changing the spacing between metal-binding motifs decreases stability and transforming activity of the human papillomavirus type 18 E7 oncoprotein.  |  Watanabe, S., et al. 1992. Virology. 190: 872-5. PMID: 1325712
2. The role of the human papillomavirus type 18 E7 oncoprotein during the complete viral life cycle.  |  McLaughlin-Drubin, ME., et al. 2005. Virology. 338: 61-8. PMID: 15921717
3. Association between poor prognosis in early-stage invasive cervical carcinomas and non-detection of HPV DNA.  |  Riou, G., et al. 1990. Lancet. 335: 1171-4. PMID: 1971033
4. Association of human papillomavirus types 16 and 18 E6 proteins with p53.  |  Werness, BA., et al. 1990. Science. 248: 76-9. PMID: 2157286
5. Detection of human papillomavirus type 18 E7 oncoprotein in cervical smears: a feasibility study.  |  Ehehalt, D., et al. 2012. J Clin Microbiol. 50: 246-57. PMID: 22135254
6. The E6 and E7 genes of the human papillomavirus type 16 together are necessary and sufficient for transformation of primary human keratinocytes.  |  Münger, K., et al. 1989. J Virol. 63: 4417-21. PMID: 2476573
7. HPV16 E6 and E7 proteins cooperate to immortalize human foreskin keratinocytes.  |  Hawley-Nelson, P., et al. 1989. EMBO J. 8: 3905-10. PMID: 2555178
8. Complex formation of human papillomavirus E7 proteins with the retinoblastoma tumor suppressor gene product.  |  Münger, K., et al. 1989. EMBO J. 8: 4099-105. PMID: 2556261
9. Development of DNA Vaccine Targeting E6 and E7 Proteins of Human Papillomavirus 16 (HPV16) and HPV18 for Immunotherapy in Combination with Recombinant Vaccinia Boost and PD-1 Antibody.  |  Peng, S., et al. 2021. mBio. 12: PMID: 33468698
10. DNA virus oncoprotein HPV18 E7 selectively antagonizes cGAS-STING-triggered innate immune activation.  |  Lou, M., et al. 2023. J Med Virol. 95: e28310. PMID: 36377393
11. Two distinct mechanisms regulate the levels of a cellular tumor antigen, p53.  |  Reich, NC., et al. 1983. Mol Cell Biol. 3: 2143-50. PMID: 6318085
12. Cloning and expression of the cDNA for E6-AP, a protein that mediates the interaction of the human papillomavirus E6 oncoprotein with p53.  |  Huibregtse, JM., et al. 1993. Mol Cell Biol. 13: 775-84. PMID: 8380895

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HPV18 E7 Antibody (F-7) has 29 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HPV18 E7 Antibody (F-7) Specifications

Alternate Product Name: Human Papillomavirus 18 E7

Product Clone Name: F-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 3-27

Epitope Target: HPV18 E7

Epitope Species: viral

Product Reactivity/Detection: HPV18 E7

Species Reactivity/Detection: n/a

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 29 citations in various scientific publications

Available Conjugates/Forms for HPV18 E7 Antibody (F-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin