HPV18 E6 Antibody (G-7): sc-365089

HPV18 E6 Antibody (G-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects HPV18 E6 protein by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-HPV18 E6 antibody (G-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HPV18 E6 plays a critical role in cervical cancer pathogenesis by promoting degradation of tumor suppressor protein p53, which regulates cell cycle and prevents tumor formation. Through p53 degradation, HPV18 E6 enables uncontrolled cell proliferation and contributes to oncogenic potential of high-risk human papillomaviruses. Approximately 90% of cervical cancers harbor high-risk HPV DNA, with HPV16 and HPV18 being most prevalent types. HPV18 E6′s interaction with p53 highlights its significance in cervical cancer development, making HPV18 E6 monoclonal antibody (G-7) valuable for studying HPV-related malignancies and molecular mechanisms of tumorigenesis.

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HPV18 E6 Antibody (G-7) References:

1. Silencing of astrin induces the p53-dependent apoptosis by suppression of HPV18 E6 expression and sensitizes cells to paclitaxel treatment in HeLa cells.  |  Yang, YC., et al. 2006. Biochem Biophys Res Commun. 343: 428-34. PMID: 16546135
2. Identification of HLA-DR1- and HLA-DR15-restricted human papillomavirus type 16 (HPV16) and HPV18 E6 epitopes recognized by CD4+ T cells from healthy young women.  |  Gallagher, KME. and Man, S. 2007. J Gen Virol. 88: 1470-1478. PMID: 17412975
3. Association between poor prognosis in early-stage invasive cervical carcinomas and non-detection of HPV DNA.  |  Riou, G., et al. 1990. Lancet. 335: 1171-4. PMID: 1971033
4. Association of human papillomavirus types 16 and 18 E6 proteins with p53.  |  Werness, BA., et al. 1990. Science. 248: 76-9. PMID: 2157286
5. The E6 and E7 genes of the human papillomavirus type 16 together are necessary and sufficient for transformation of primary human keratinocytes.  |  Münger, K., et al. 1989. J Virol. 63: 4417-21. PMID: 2476573
6. HPV16 E6 and E7 proteins cooperate to immortalize human foreskin keratinocytes.  |  Hawley-Nelson, P., et al. 1989. EMBO J. 8: 3905-10. PMID: 2555178
7. Complex formation of human papillomavirus E7 proteins with the retinoblastoma tumor suppressor gene product.  |  Münger, K., et al. 1989. EMBO J. 8: 4099-105. PMID: 2556261
8. Augmentation of cellular and humoral immune responses to HPV16 and HPV18 E6 and E7 antigens by VGX-3100.  |  Morrow, MP., et al. 2016. Mol Ther Oncolytics. 3: 16025. PMID: 28054033
9. Irreversible electroporation-mediated shRNA knockdown of the HPV18 E6 gene suppresses cervical cancer growth in vitro and in vivo.  |  Wang, ZL., et al. 2017. Oncol Lett. 14: 1943-1949. PMID: 28781638
10. A Phase 1 Trial Assessing the Safety and Tolerability of a Therapeutic DNA Vaccination Against HPV16 and HPV18 E6/E7 Oncogenes After Chemoradiation for Cervical Cancer.  |  Hasan, Y., et al. 2020. Int J Radiat Oncol Biol Phys. 107: 487-498. PMID: 32151670
11. Two distinct mechanisms regulate the levels of a cellular tumor antigen, p53.  |  Reich, NC., et al. 1983. Mol Cell Biol. 3: 2143-50. PMID: 6318085
12. Cloning and expression of the cDNA for E6-AP, a protein that mediates the interaction of the human papillomavirus E6 oncoprotein with p53.  |  Huibregtse, JM., et al. 1993. Mol Cell Biol. 13: 775-84. PMID: 8380895

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HPV18 E6 Antibody (G-7) has 38 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HPV18 E6 Antibody (G-7) Specifications

Alternate Product Name: HPV18 E6

Product Clone Name: G-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 4-31

Epitope Target: HPV18 E6

Epitope Species: viral

Product Reactivity/Detection: HPV18 E6

Species Reactivity/Detection: n/a

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 38 citations in various scientific publications

Available Conjugates/Forms for HPV18 E6 Antibody (G-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred HPV16 E6/18 E6 antibody is HPV16 E6/18 E6 Antibody (C1P5): sc-460.