HPV16 L2 Antibody (2JGmab#5): sc-65709

HPV16 L2 (2JGmab#5) is a mouse monoclonal IgG1 kappa light chain antibody that detects HPV16 L2 protein of HPV-16 origin by western blotting (WB) and immunofluorescence (IF). HPV16 L2 (2JGmab#5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HPV16 L2 protein plays a crucial role in the viral life cycle, particularly in the assembly and stability of the viral capsid. HPV16 L2 protein interacts with the major capsid protein L1, facilitating the encapsidation of the viral genome during the assembly process. L2 binding to L1 is essential for proper capsid formation, ensuring efficient viral DNA packaging and protection. L1-L2 interaction is vital for viral infection of host cells, as L1 protein forms the outer shell while L2 participates in internal processes necessary for viral replication. Understanding L1-L2 interaction dynamics reveals HPV16′s pathogenic mechanisms and highlights potential therapeutic targets for preventing HPV-related diseases, including cervical cancer.

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HPV16 L2 Antibody (2JGmab#5) References:

1. Common neutralization epitope in minor capsid protein L2 of human papillomavirus types 16 and 6.  |  Kawana, K., et al. 1999. J Virol. 73: 6188-90. PMID: 10364381
2. Nasal immunization of mice with human papillomavirus type 16 (HPV-16) virus-like particles or with the HPV-16 L1 gene elicits specific cytotoxic T lymphocytes in vaginal draining lymph nodes.  |  Dupuy, C., et al. 1999. J Virol. 73: 9063-71. PMID: 10516012
3. The human papillomavirus type 16 negative regulatory RNA element interacts with three proteins that act at different posttranscriptional levels.  |  Koffa, MD., et al. 2000. Proc Natl Acad Sci U S A. 97: 4677-82. PMID: 10781073
4. Structure of small virus-like particles assembled from the L1 protein of human papillomavirus 16.  |  Chen, XS., et al. 2000. Mol Cell. 5: 557-67. PMID: 10882140
5. The nine C-terminal amino acids of the major capsid protein of the human papillomavirus type 16 are essential for DNA binding and gene transfer capacity.  |  Touzé, A., et al. 2000. FEMS Microbiol Lett. 189: 121-7. PMID: 10913877
6. Mucosal vaccination with a recombinant Salmonella typhimurium expressing human papillomavirus type 16 (HPV16) L1 virus-like particles (VLPs) or HPV16 VLPs purified from insect cells inhibits the growth of HPV16-expressing tumor cells in mice.  |  Revaz, V., et al. 2001. Virology. 279: 354-60. PMID: 11145916
7. Human papillomavirus type 16 minor capsid protein l2 N-terminal region containing a common neutralization epitope binds to the cell surface and enters the cytoplasm.  |  Kawana, Y., et al. 2001. J Virol. 75: 2331-6. PMID: 11160736
8. L1 interaction domains of papillomavirus l2 necessary for viral genome encapsidation.  |  Okun, MM., et al. 2001. J Virol. 75: 4332-42. PMID: 11287582
9. Vaccine regimen for prevention of sexually transmitted infections with human papillomavirus type 16.  |  Kowalczyk, DW., et al. 2001. Vaccine. 19: 3583-90. PMID: 11348726
10. Nuclear localization but not PML protein is required for incorporation of the papillomavirus minor capsid protein L2 into virus-like particles.  |  Becker, KA., et al. 2004. J Virol. 78: 1121-8. PMID: 14722267
11. A pan-HPV vaccine based on bacteriophage PP7 VLPs displaying broadly cross-neutralizing epitopes from the HPV minor capsid protein, L2.  |  Tumban, E., et al. 2011. PLoS One. 6: e23310. PMID: 21858066
12. The BICD2 dynein cargo adaptor binds to the HPV16 L2 capsid protein and promotes HPV infection.  |  Speckhart, K., et al. 2024. PLoS Pathog. 20: e1012289. PMID: 38829892

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HPV16 L2 Antibody (2JGmab#5) has 4 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HPV16 L2 Antibody (2JGmab#5) Specifications

Alternate Product Name: HPV16 L2

Product Clone Name: 2JGmab#5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 40-150

Epitope Target: HPV16 L2

Epitope Species: HPV16

Product Reactivity/Detection: HPV16 L2

Species Reactivity/Detection: HPV-16

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for HPV16 L2 Antibody (2JGmab#5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin