Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HoxD3 Double Nickase Plasmid (h): sc-405920-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HoxD3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HoxD3 Double Nickase Plasmid (h) and HoxD3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HOXD3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HoxD3 Antibody (4AY): sc-130378
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HoxD3 Double Nickase Plasmid (h) | sc-405920-NIC | 20 µg | $410.00 | |||
HoxD3 Double Nickase Plasmid (h2) | sc-405920-NIC-2 | 20 µg | $410.00 |
HOXD3 encodes the human homeobox transcription factor HoxD3, a DNA-binding regulator that contributes to anterior–posterior patterning and positional identity during development. Through sequence-specific transcriptional control, HoxD3 influences programs linked to morphogenesis, cell migration, and lineage specification, integrating with broader HOX-regulated developmental gene networks. Dysregulated HOXD3 expression has been associated with altered differentiation states and aberrant invasive or angiogenic phenotypes reported in multiple tumor biology contexts, making it relevant for studying transcriptional circuitry in disease-associated cellular plasticity. As a nuclear transcription factor, HoxD3 is commonly investigated for its downstream targets, chromatin-dependent regulation, and context-specific effects on cell fate decisions.
HoxD3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HOXD3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HOXD3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HOXD3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HOXD3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.