HoxD12 CRISPR/Cas9 KO Plasmid (h): sc-407472
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HoxD12 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HoxD12 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HoxD12 CRISPR/Cas9 KO Plasmid (h) | sc-407472 | 20 µg | $397.00 |
Overview
HOXD12 encodes the homeobox transcription factor HoxD12, a member of the HOXD cluster that helps establish positional identity during embryonic development and coordinates patterning of the limb and axial skeleton. As a DNA-binding regulator, HoxD12 integrates with broader HOX regulatory networks and chromatin-level control to shape tissue-specific transcriptional programs governing differentiation and morphogenesis. Dysregulated HOXD12 expression or perturbation of HOX cluster regulation has been associated with developmental anomalies and has been reported in transcriptional signatures across multiple cancers, reflecting its role in controlling lineage and growth-related gene networks. In adult and disease contexts, altered HOXD12 activity can influence cell state transitions, migration programs, and developmental pathway reactivation.
HoxD12 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HOXD12 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HOXD12 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HOXD12 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HoxD12 protein expression.
This CRISPR knockout system enables efficient generation of HOXD12-deficient cell models for investigation of HoxD12 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HOXD12 exon(s) critical for HoxD12 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HOXD12 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HoxD12 CRISPR/Cas9 KO Plasmid (h) and HoxD12 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HOXD12 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HoxD12 HDR Plasmid (h) and HoxD12 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HOXD12 homology arms to support homology-directed repair at defined HOXD12 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.