HoxD10 Double Nickase Plasmid (h): sc-403328-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HoxD10 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HoxD10 Double Nickase Plasmid (h) and HoxD10 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HOXD10. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HoxD10 Antibody (G-3): sc-166235
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HoxD10 Double Nickase Plasmid (h) | sc-403328-NIC | 20 µg | $410.00 | |||
HoxD10 Double Nickase Plasmid (h2) | sc-403328-NIC-2 | 20 µg | $410.00 |
HOXD10 encodes the homeobox transcription factor HoxD10, a sequence-specific DNA-binding regulator that helps establish positional identity and differentiation programs during embryonic development. In human cells, HoxD10 influences transcriptional networks involved in morphogenesis, cell adhesion, and cytoskeletal organization, integrating with broader HOX patterning pathways and downstream effectors that shape tissue architecture. Dysregulated HOXD10 expression or altered regulatory circuitry has been reported in multiple tumor contexts, where it is frequently studied for links to epithelial–mesenchymal transition, invasion-associated gene expression, and metastatic behavior. As a developmental regulator with context-dependent effects, HOXD10 is also used to interrogate how transcriptional programs are rewired across differentiation and disease-relevant states.
HoxD10 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HOXD10 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HOXD10. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HOXD10 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HOXD10-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.