HoxC9 Double Nickase Plasmid (h): sc-404568-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HoxC9 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HoxC9 Double Nickase Plasmid (h) and HoxC9 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HOXC9. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HoxC9 Antibody (HOXCA6E6): sc-81100
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HoxC9 Double Nickase Plasmid (h) | sc-404568-NIC | 20 µg | $410.00 | |||
HoxC9 Double Nickase Plasmid (h2) | sc-404568-NIC-2 | 20 µg | $410.00 |
HOXC9 encodes the homeobox transcription factor HoxC9, a sequence-specific DNA-binding regulator that patterns the anterior–posterior axis during embryogenesis and guides neural and mesodermal differentiation programs. In human cells, HoxC9 influences chromatin-state and transcriptional networks controlling lineage specification, neuronal maturation, and regional identity through HOX cluster regulatory circuitry and cofactor-dependent transcriptional complexes. Dysregulated HOXC9 expression has been reported in multiple tumor contexts, where altered HOX-driven gene programs can affect proliferation, migration, and differentiation states. As a developmental regulator with context-dependent oncogenic or tumor-suppressive associations, HOXC9 is frequently studied for its role in cell fate transitions and disease-relevant transcriptional remodeling.
HoxC9 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HOXC9 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HOXC9. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HOXC9 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HOXC9-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.