HoxC4 Double Nickase Plasmid (h): sc-405942-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HoxC4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HoxC4 Double Nickase Plasmid (h) and HoxC4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HOXC4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HoxC4 Antibody (G-12): sc-398460
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HoxC4 Double Nickase Plasmid (h) | sc-405942-NIC | 20 µg | $410.00 |
HOXC4 encodes the homeobox transcription factor HoxC4, a DNA-binding regulator that helps establish positional identity and coordinate gene expression programs during embryonic patterning and tissue differentiation. As part of HOX-dependent transcriptional networks, HoxC4 influences chromatin and transcriptional states that govern lineage commitment, proliferation, and morphogenesis. Dysregulated HOXC4 expression has been associated with altered differentiation states and transcriptional rewiring observed in developmental disorders and multiple cancer contexts, supporting its use as a molecular node in studies of oncogenic gene regulation. Human HOXC4 is also studied for its interactions with cofactors that modulate promoter/enhancer activity, enabling pathway-level interrogation of homeobox-driven regulatory circuits.
HoxC4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HOXC4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HOXC4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HOXC4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HOXC4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.