HoxC10 CRISPR/Cas9 KO Plasmid (m): sc-431637
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HoxC10 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HoxC10 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HoxC10 CRISPR/Cas9 KO Plasmid (m) | sc-431637 | 20 µg | $397.00 |
Overview
Hoxc10 encodes the homeobox transcription factor HoxC10, a sequence-specific DNA-binding regulator that helps establish anterior–posterior patterning and positional identity during embryogenesis. In mouse development, HOX programs integrate with morphogen-driven signaling networks such as WNT, FGF, retinoic acid, and BMP pathways to coordinate lineage specification, axial skeletal formation, and regional differentiation. Beyond development, altered HOX gene regulation is commonly linked to dysregulated transcriptional networks that influence cell fate maintenance, migration, and differentiation. Perturbation of HOXC10-associated programs is therefore relevant for studying congenital patterning defects and transcriptional reprogramming mechanisms implicated in disease biology.
HoxC10 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Hoxc10 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Hoxc10 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Hoxc10 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HoxC10 protein expression.
This CRISPR knockout system enables efficient generation of Hoxc10-deficient cell models for investigation of HoxC10 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Hoxc10 exon(s) critical for HoxC10 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Hoxc10 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HoxC10 CRISPR/Cas9 KO Plasmid (m) and HoxC10 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Hoxc10 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HoxC10 HDR Plasmid (m) and HoxC10 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Hoxc10 homology arms to support homology-directed repair at defined Hoxc10 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.