Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HoxB9 Double Nickase Plasmid (h): sc-401770-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HoxB9 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HoxB9 Double Nickase Plasmid (h) and HoxB9 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HOXB9. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HoxB9 Antibody (H-8): sc-398500
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HoxB9 Double Nickase Plasmid (h) | sc-401770-NIC | 20 µg | $410.00 | |||
HoxB9 Double Nickase Plasmid (h2) | sc-401770-NIC-2 | 20 µg | $410.00 |
HOXB9 encodes the homeobox transcription factor HoxB9, a DNA-binding regulator that helps establish anterior–posterior patterning and tissue identity during embryogenesis. In human cells, HoxB9 influences lineage specification and differentiation programs by modulating transcriptional networks linked to morphogenesis, cell-cycle control, and epithelial–mesenchymal plasticity. Dysregulated HOXB9 expression has been associated with oncogenic transcriptional states in multiple tumor contexts, including effects on angiogenic and invasive gene signatures, and it is also studied in developmental disorders involving altered patterning. As a node within homeobox gene regulatory circuits, HoxB9 is relevant for dissecting developmental gene networks and transcriptional control of cell fate.
HoxB9 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HOXB9 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HOXB9. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HOXB9 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HOXB9-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.