HoxB5 CRISPR/Cas9 KO Plasmid (h): sc-409631
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HoxB5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HoxB5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HoxB5 Antibody (133C3a): sc-81099
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HoxB5 CRISPR/Cas9 KO Plasmid (h) | sc-409631 | 20 µg | $397.00 |
Overview
HOXB5 encodes the homeobox transcription factor HoxB5, a sequence-specific DNA-binding regulator that helps establish anterior–posterior patterning and tissue identity during embryonic development. HoxB5 participates in transcriptional programs downstream of developmental morphogen signaling, including retinoic acid–responsive pathways, to coordinate lineage specification, proliferation, and differentiation. In adult contexts, dysregulated HOXB5 expression has been associated with altered cell state control and aberrant transcriptional networks observed across multiple cancer and hematopoietic disease datasets. As a nuclear regulator, HoxB5 is commonly studied to map gene regulatory circuits, enhancer–promoter logic, and developmental reprogramming mechanisms in human cell models.
HoxB5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HOXB5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HOXB5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HOXB5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HoxB5 protein expression.
This CRISPR knockout system enables efficient generation of HOXB5-deficient cell models for investigation of HoxB5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HOXB5 exon(s) critical for HoxB5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HOXB5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HoxB5 CRISPR/Cas9 KO Plasmid (h) and HoxB5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HOXB5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HoxB5 HDR Plasmid (h) and HoxB5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HOXB5 homology arms to support homology-directed repair at defined HOXB5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.