Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HoxB13 Double Nickase Plasmid (h): sc-401878-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HoxB13 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HoxB13 Double Nickase Plasmid (h) and HoxB13 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HOXB13. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HoxB13 Antibody (F-9): sc-28333
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HoxB13 Double Nickase Plasmid (h) | sc-401878-NIC | 20 µg | $410.00 | |||
HoxB13 Double Nickase Plasmid (h2) | sc-401878-NIC-2 | 20 µg | $410.00 |
HOXB13 encodes the homeobox transcription factor HoxB13, a sequence-specific DNA-binding regulator that helps establish anterior–posterior patterning and controls differentiation programs in urogenital and prostate lineages. By modulating transcriptional networks downstream of developmental signaling pathways, HoxB13 influences cell fate decisions, epithelial maturation, and tissue-specific gene expression. Dysregulated HOXB13 activity and altered transcriptional circuitry have been linked to prostate biology and oncogenic reprogramming, making it a relevant target for studying lineage plasticity and tumor-associated gene regulatory states. In biomedical research, HOXB13 is commonly investigated for its roles in transcriptional control, chromatin-dependent regulation, and context-dependent effects on proliferation and differentiation.
HoxB13 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HOXB13 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HOXB13. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HOXB13 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HOXB13-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.