HoxA5 Double Nickase Plasmid (h): sc-402499-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HoxA5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HoxA5 Double Nickase Plasmid (h) and HoxA5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HOXA5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HoxA5 Antibody (C-11): sc-365784
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HoxA5 Double Nickase Plasmid (h) | sc-402499-NIC | 20 µg | $410.00 | |||
HoxA5 Double Nickase Plasmid (h2) | sc-402499-NIC-2 | 20 µg | $410.00 |
HOXA5 encodes the homeobox transcription factor HoxA5, a sequence-specific DNA-binding regulator that helps establish anterior–posterior patterning and coordinates programs of cellular differentiation during development. In adult tissues, HoxA5 influences transcriptional networks controlling epithelial–mesenchymal state, extracellular matrix organization, and context-dependent cell-cycle and apoptotic responses. As part of HOX gene regulatory circuitry, it integrates with chromatin and lineage-specifying pathways to shape tissue identity and remodeling. Dysregulated HOXA5 expression or epigenetic silencing has been associated with altered differentiation states and tumor-associated transcriptional reprogramming in several tissue contexts, supporting its use as a mechanistic node in disease-model research.
HoxA5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HOXA5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HOXA5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HOXA5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HOXA5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.