Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HoxA4 Double Nickase Plasmid (h): sc-405914-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HoxA4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HoxA4 Double Nickase Plasmid (h) and HoxA4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HOXA4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HoxA4 Antibody (H-4): sc-515418
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HoxA4 Double Nickase Plasmid (h) | sc-405914-NIC | 20 µg | $410.00 | |||
HoxA4 Double Nickase Plasmid (h2) | sc-405914-NIC-2 | 20 µg | $410.00 |
HOXA4 encodes the homeobox transcription factor HoxA4, a DNA-binding regulator that helps establish anterior–posterior patterning and maintains positional identity during embryogenesis. In human cells, HoxA4 modulates transcriptional programs linked to differentiation, proliferation, and lineage commitment, integrating with developmental signaling and chromatin regulatory networks. HOXA4 activity is closely tied to HOX cluster regulation, including epigenetic control by Polycomb/Trithorax complexes, and contributes to tissue-specific gene expression in hematopoietic and mesenchymal contexts. Dysregulated HOXA4 expression has been associated with altered developmental gene signatures and is studied in relation to oncogenic transcriptional states and aberrant differentiation.
HoxA4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HOXA4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HOXA4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HOXA4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HOXA4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.