Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HoxA3 Double Nickase Plasmid (h): sc-404634-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HoxA3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HoxA3 Double Nickase Plasmid (h) and HoxA3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HOXA3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HoxA3 Antibody (F-7): sc-374237
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HoxA3 Double Nickase Plasmid (h) | sc-404634-NIC | 20 µg | $410.00 | |||
HoxA3 Double Nickase Plasmid (h2) | sc-404634-NIC-2 | 20 µg | $410.00 |
HOXA3 encodes the homeobox transcription factor HoxA3, a sequence-specific DNA-binding regulator that helps establish anterior–posterior patterning and regional identity during embryonic development. HoxA3 coordinates gene expression programs controlling morphogenesis, cell migration, and differentiation, integrating with broader HOX regulatory networks and chromatin-mediated transcriptional control. Dysregulated HOXA3 expression or altered HOX cluster regulation has been implicated in developmental abnormalities and contributes to oncogenic transcriptional states in multiple tumor contexts. In human cell models, HOXA3 is commonly studied to map lineage-specifying circuits, investigate transcriptional control of developmental pathways, and characterize phenotypic outcomes of HOX network perturbation.
HoxA3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HOXA3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HOXA3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HOXA3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HOXA3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.