Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HoxA10 Double Nickase Plasmid (h): sc-401463-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HoxA10 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HoxA10 Double Nickase Plasmid (h) and HoxA10 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HOXA10. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HoxA10 Antibody (E-11): sc-271428
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HoxA10 Double Nickase Plasmid (h) | sc-401463-NIC | 20 µg | $410.00 | |||
HoxA10 Double Nickase Plasmid (h2) | sc-401463-NIC-2 | 20 µg | $410.00 |
HOXA10 encodes the homeobox transcription factor HoxA10, a sequence-specific DNA-binding regulator that orchestrates embryonic patterning and adult tissue homeostasis through control of lineage-determining gene networks. In hematopoietic and mesenchymal contexts, HoxA10 integrates developmental signaling programs to modulate proliferation, differentiation, adhesion, and extracellular matrix remodeling, with downstream effects on cell-cycle and transcriptional regulation pathways. Dysregulated HOXA10 expression or altered regulatory circuitry has been linked to perturbed myeloid maturation and broader transcriptional reprogramming observed in malignancy-associated states, making it a useful node for studying developmental gene control in disease-relevant models. In reproductive biology, HOXA10 contributes to endometrial differentiation and uterine receptivity programs, supporting investigations into hormone-responsive transcriptional networks.
HoxA10 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HOXA10 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HOXA10. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HOXA10 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HOXA10-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.