HoxA1 Double Nickase Plasmid (h): sc-403158-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HoxA1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HoxA1 Double Nickase Plasmid (h) and HoxA1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HOXA1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HoxA1 Antibody (1E10): sc-293257
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HoxA1 Double Nickase Plasmid (h) | sc-403158-NIC | 20 µg | $410.00 | |||
HoxA1 Double Nickase Plasmid (h2) | sc-403158-NIC-2 | 20 µg | $410.00 |
HOXA1 encodes the homeobox transcription factor HoxA1, an early developmental regulator that controls anterior–posterior patterning and segment identity by binding DNA through its homeodomain and coordinating transcriptional programs with cofactors such as PBX and MEIS. HoxA1 influences cell fate decisions, differentiation timing, and morphogen-responsive gene networks, integrating with signaling pathways including retinoic acid, WNT, and FGF during embryogenesis. Dysregulated HOXA1 expression or genetic variation has been linked to congenital developmental phenotypes and has been reported in multiple disease-associated transcriptional signatures, supporting its relevance for studying lineage specification and aberrant gene regulation.
HoxA1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HOXA1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HOXA1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HOXA1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HOXA1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.