RNAi-directed mRNA Cleavage
HOTTL siRNA and shRNA Plasmids (h)
- Target species: human
- Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length
- For independent verification of HOTTL gene silencing results, individual duplex components or plasmids are also available upon request
- Suitable control antibody: HOTTL Antibody (F-8): sc-166483 is recommended as control antibody for monitoring of HOTTL expression knockdown by Western blotting or immunofluorescence
- Get details about si/shRNA Gene Silencers
- Gene silencers are intended for noncommercial and research use only. Click here for details.
QUICK LINKS
Ordering Information
siRNA
Primer
shRNA Plasmid
shRNA Lentiviral Particles
Recommended Control Antibody
SEE ALSO...
HOTTL Antibodies for analysis of cellular responses to HOTTL si/shRNA Gene Silencers
Description
- 10 µM, 50-100 transfections
- siRNA products generally consist of pools of three to five target-specific 19-25 nt siRNAs designed to knockdown gene expression
- for independent verification of HOTTL gene silencing results, individual siRNA duplex components are also available upon request
- suitable control antibody: HOTTL Antibody (F-8): sc-166483
- sqRT-PCR Primer also available: HOTTL (h)-PR: sc-62474-PR, 20 µl
- Get details about si/shRNA Gene Silencers
See Protocol Online.
DOWNLOADS
QUICK LINKS
Related Products
Description
- 20 µl of each primer set is provided at a concentration of 10 µM
- HOTTL Primer (h) includes A set (forward & reverse) and B set (forward & reverse) primers
- Primer sets are recommended for semi-quantitative nested reverse transcriptase PCR
See Protocol Online.
DOWNLOADS
QUICK LINKS
Related Products
Description
- 20 µg, up to 20 transfections
- shRNA Plasmids generally consist of a pool of three to five lentiviral vector plasmids each encoding target-specific 19-25 nt (plus hairpin) shRNAs designed to knockdown gene expression
- shRNA sequences correspond to HOTTL siRNA Gene Silencer sequences
- provided as transfection-ready purified plasmid DNA target-specific gene silencing
- after transfection, cells stably expressing shRNAs can be isolated by puromycin selection
- shRNA Plasmids are intended only for non-commercial use.
- suitable control antibody: HOTTL Antibody (F-8): sc-166483
- sqRT-PCR Primer also available: HOTTL (h)-PR: sc-62474-PR, 20 µl
See Protocol Online.
DOWNLOADS
QUICK LINKS
Related Products
Description
- 200 µl viral stock containing 1x 106 infectious units of virus (IFU) , sufficient for 10-20 transductions
- Lentiviral Particles are provided as transduction-ready viral particles for gene silencing
- Lentiviral Particles generally contain three to five expression constructs each encoding target-specific 19-25 nt (plus hairpin) shRNA designed to knockdown gene expression
- shRNA sequences correspond to HOTTL siRNA Gene Silencer sequences
- After transduction, stable cell lines expressing the shRNA may be isolated via selection with puromycin
- Biosafety - Lentiviral Particles are replication-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells.
- Lentiviral Particles are intended for non-commercial use.
See Protocol Online.
DOWNLOADS
QUICK LINKS
Related Products
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HOTTL siRNA (h) | sc-62474 | 10 µM | $298.00 | |||
HOTTL (h)-PR | sc-62474-PR | 10 µM, 20 µl | $34.00 | |||
HOTTL shRNA Plasmid (h) | sc-62474-SH | 20 µg | $603.00 | |||
HOTTL shRNA (h) Lentiviral Particles | sc-62474-V | 200 µl | $725.00 |