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Horseradish Peroxidase Antibody (HP-03): sc-51625

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Datasheets
  • Horseradish Peroxidase Antibody (HP-03) is a mouse monoclonal IgG1 Horseradish Peroxidase antibody, cited in 5 publications, provided at 100 µg/ml
  • raised against horseradish peroxidase (Grade I)
  • recommended for detection of horseradish peroxidase of Armoracia rusticana origin by WB and IF
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Horseradish Peroxidase Antibody (HP-03). This work is in progress.

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Horseradish Peroxidase Antibody (HP-03) is a mouse monoclonal IgG1 antibody that detects horseradish peroxidase (HRP) protein of Armoracia rusticana origin by western blotting (WB) and immunofluorescence (IF). Anti-HRP antibody (HP-03) is available as the non-conjugated format. HRP (HP-03) antibody plays a crucial role in various biochemical processes, particularly in the oxidation of small aromatic substrates, which is essential for plant metabolism and the synthesis of important compounds such as lignin and plant hormones. HRP (HP-03) monoclonal antibody catalyzes these reactions using hydrogen peroxide as an oxidizing agent, highlighting its significance in plant physiology and biotechnological applications. HRP′s structure as a single chain polypeptide with four disulfide bridges contributes to its stability and functionality, making HRP (HP-03) antibody an invaluable tool in research and diagnostic applications. Anti-HRP antibody (HP-03) is widely utilized in immunohistochemistry with paraffin-embedded sections (IHCP) for labeling antigens and their corresponding antibodies, providing a non-cytotoxic means to visualize cellular components.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Horseradish Peroxidase Antibody (HP-03) References:

  1. Development of a chemiluminescence-based ribonuclease protection assay.  |  Rosenau, C., et al. 2002. Biotechniques. 33: 1354-8. PMID: 12503324
  2. The endogenous calcium ions of horseradish peroxidase C are required to maintain the functional nonplanarity of the heme.  |  Laberge, M., et al. 2003. Biophys J. 84: 2542-52. PMID: 12668462
  3. Vesicular transport route of horseradish C1a peroxidase is regulated by N- and C-terminal propeptides in tobacco cells.  |  Matsui, T., et al. 2003. Appl Microbiol Biotechnol. 62: 517-22. PMID: 12879300
  4. The role of stigma peroxidases in flowering plants: insights from further characterization of a stigma-specific peroxidase (SSP) from Senecio squalidus (Asteraceae).  |  McInnis, SM., et al. 2006. J Exp Bot. 57: 1835-46. PMID: 16698818
  5. Horseradish peroxidase uptake and crinophagy in insulin-secreting cells.  |  Sawano, F., et al. 1986. Exp Cell Res. 164: 174-82. PMID: 3514252
  6. Preparation of peroxidase: antiperoxidase (PAP) complexes for immunohistological labeling of monoclonal antibodies.  |  Mason, DY., et al. 1982. J Histochem Cytochem. 30: 1114-22. PMID: 6183312
  7. Monoclonal antibodies against horseradish peroxidase.  |  Viklický, V., et al. 1984. Folia Biol (Praha). 30: 377-82. PMID: 6394370

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Horseradish Peroxidase Antibody (HP-03)

sc-51625
100 µg/ml
$316.00

In all added paper sc-51625 Abs are described as "HRP-conjugated secondary antibody". On website sc-51625 Abs are described as monoclonal antibodies against HRP. Please provide which information is correct?

Asked by: Anton1
Thank you for your question. This antibody is specific against Horseradish peroxidase protein. It is not conjugated and does not recognize other primary antibodies.
Answered by: Tech Support Europe
Date published: 2024-08-10

What is the total volume provided of Horseradish Peroxidase (HP-03): sc-51625 monoclonal antibody?

Asked by: Cweed
Thank you for your question. This antibody is provided as 100 µg in 1 ml total volume.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by from Good for Western BlotThis antibody has been used for western blotting in published research. -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from Dr SadekAb work good with dilution 1:200 and 2 hrs incubation at 37ºc and overnight at 4 ºc
Date published: 2018-11-21
Rated 4 out of 5 by from WB publishable data with BV2 mouse microglialWB publishable data with BV2 mouse microglial cell line - SCBT Publication Review
Date published: 2015-04-03
Rated 4 out of 5 by from Successful application of this antibody inSuccessful application of this antibody in WB using human AGS and N87 Gastric Cancer Cells -SCBT Publication Review
Date published: 2015-04-01
Rated 4 out of 5 by from This antibody was used in a publication forThis antibody was used in a publication for WB on rat amacrine cells - SCBT Publication Review
Date published: 2015-01-19
Rated 4 out of 5 by from Publishable WB data with human HEK293Publishable WB data with human HEK293, U2OS and Caco-2 - SCBT Publication Review
Date published: 2013-02-04
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Horseradish Peroxidase Antibody (HP-03) is rated 4.3 out of 5 by 6.
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