Hog1 Antibody (F-9): sc-365609
- Hog1 Antibody (F-9) is a mouse monoclonal IgM κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 389-421 near the C-terminus of Hog1 of Saccharomyces cerevisiae origin
- recommended for detection of Hog1 of y origin by WB, IP, IF and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Hog1 Antibody (F-9). This work is in progress.
QUICK LINKS
SEE ALSO...
Hog1 Antibody (F-9) is a mouse monoclonal IgM antibody that detects Hog1 in yeast species by applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Hog1, also known as Ssk3, plays a crucial role in the cellular response to high osmolarity, acting as a key component of the mitogen-activated protein kinase (MAPK) signaling cascade. This cascade is vital for transmitting extracellular signals to the cytoplasm and nucleus, allowing yeast cells to adapt to environmental changes. The activation of Hog1 is initiated by the upstream MAPKK Pbs2, which responds to increased external osmolarity. Once activated, Hog1 functions as a transcription factor that upregulates the expression of osmo-regulatory proteins, thereby helping the cell maintain osmotic balance and survive under stress conditions. The ability of Hog1 to modulate gene expression in response to osmotic stress underscores its importance in cellular homeostasis and adaptation, making anti-Hog1 antibody (F-9) an essential tool for researchers studying stress response mechanisms in yeast.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Hog1 Antibody (F-9) References:
- Rck2 kinase is a substrate for the osmotic stress-activated mitogen-activated protein kinase Hog1. | Bilsland-Marchesan, E., et al. 2000. Mol Cell Biol. 20: 3887-95. PMID: 10805732
- The MAPK Hog1 recruits Rpd3 histone deacetylase to activate osmoresponsive genes. | De Nadal, E., et al. 2004. Nature. 427: 370-4. PMID: 14737171
- Hog1 mediates cell-cycle arrest in G1 phase by the dual targeting of Sic1. | Escoté, X., et al. 2004. Nat Cell Biol. 6: 997-1002. PMID: 15448699
- Tighter αC-helix-αL16-helix interactions seem to make p38α less prone to activation by autophosphorylation than Hog1. | Tesker, M., et al. 2016. Biosci Rep. 36: PMID: 26987986
- Stress-induced nuclear accumulation is dispensable for Hog1-dependent gene expression and virulence in a fungal pathogen. | Day, AM., et al. 2017. Sci Rep. 7: 14340. PMID: 29085028
- The MAP-Kinase HOG1 Controls Cold Adaptation in Rhodosporidium kratochvilovae by Promoting Biosynthesis of Polyunsaturated Fatty Acids and Glycerol. | Chen, W., et al. 2022. Curr Microbiol. 79: 253. PMID: 35834133
- Host- and virus-induced gene silencing of HOG1-MAPK cascade genes in Rhizophagus irregularis inhibit arbuscule development and reduce resistance of plants to drought stress. | Wang, S., et al. 2023. Plant Biotechnol J. 21: 866-883. PMID: 36609693
- White Collar 1 Modulates Oxidative Sensitivity and Virulence by Regulating the HOG1 Pathway in Fusarium asiaticum. | Tang, Y., et al. 2023. Microbiol Spectr. 11: e0520622. PMID: 37195224
- The Mitogen-Activated Protein Kinase Hog1 Regulates Fungal Development, Pathogenicity, and Stress Response in Botryosphaeria dothidea. | Zhang, Y., et al. 2024. Phytopathology. 114: 725-731. PMID: 37889135
- Gcn2 rescues reprogramming in the absence of Hog1/p38 signaling in C. neoformans during thermal stress. | Goich, D., et al. 2024. mBio. e0176224. PMID: 39670714
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hog1 Antibody (F-9) | sc-365609 | 200 µg/ml | $322.00 | |||
Hog1 (F-9) Neutralizing Peptide | sc-365609 P | 100 µg/0.5 ml | $69.00 |