hnRNP Q Antibody (I8E4): sc-56703

hnRNP Q Antibody (I8E4) is a mouse monoclonal IgG1 kappa light chain antibody that detects hnRNP Q protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). anti-hnRNP Q antibody (I8E4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The heterogeneous nuclear ribonucleoprotein Q (hnRNP Q) plays a pivotal role in RNA processing, particularly in regulating pre-mRNA splicing, which is essential for proper gene expression. hnRNP Q is widely expressed across different tissues and is developmentally regulated, highlighting its importance in various biological processes. hnRNP Q interacts with several key proteins, including survival motor neuron protein (SMN), which is crucial for motor neuron health. Disruption of hnRNP Q and SMN interaction due to genetic mutations can lead to spinal muscular atrophy, a severe neurodegenerative disorder. Additionally, hnRNP Q exhibits a unique expression pattern, being upregulated after midnight, which correlates with a decline in AANAT, the enzyme responsible for melatonin synthesis. This rhythmic regulation of AANAT mRNA degradation by hnRNP Q suggests a significant role in circadian rhythm modulation, further emphasizing hnRNP Q′s importance in both RNA processing and biological rhythm maintenance.

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hnRNP Q Antibody (I8E4) References:

1. Two-dimensional electrophoresis and mass spectrometry identification of proteins bound by a murine monoclonal anti-cardiolipin antibody: a powerful technique to characterize the cross-reactivity of a single autoantibody.  |  Thebault, S., et al. 2000. Electrophoresis. 21: 2531-9. PMID: 10939468
2. SMN interacts with a novel family of hnRNP and spliceosomal proteins.  |  Mourelatos, Z., et al. 2001. EMBO J. 20: 5443-52. PMID: 11574476
3. Specific interaction of Smn, the spinal muscular atrophy determining gene product, with hnRNP-R and gry-rbp/hnRNP-Q: a role for Smn in RNA processing in motor axons?  |  Rossoll, W., et al. 2002. Hum Mol Genet. 11: 93-105. PMID: 11773003
4. Hyperphosphorylated C-terminal repeat domain-associating proteins in the nuclear proteome link transcription to DNA/chromatin modification and RNA processing.  |  Carty, SM. and Greenleaf, AL. 2002. Mol Cell Proteomics. 1: 598-610. PMID: 12376575
5. Evidence for a modifying pathway in SMA discordant families: reduced SMN level decreases the amount of its interacting partners and Htra2-beta1.  |  Helmken, C., et al. 2003. Hum Genet. 114: 11-21. PMID: 14520560
6. Transforming growth factor-beta1-regulated proteins in human endothelial cells identified by two-dimensional gel electrophoresis and mass spectrometry.  |  Lomnytska, M., et al. 2004. Proteomics. 4: 995-1006. PMID: 15048981
7. HRP-2, a heterogeneous nuclear ribonucleoprotein, is essential for embryogenesis and oogenesis in Caenorhabditis elegans.  |  Kinnaird, JH., et al. 2004. Exp Cell Res. 298: 418-30. PMID: 15265690
8. Rhythmic serotonin N-acetyltransferase mRNA degradation is essential for the maintenance of its circadian oscillation.  |  Kim, TD., et al. 2005. Mol Cell Biol. 25: 3232-46. PMID: 15798208
9. Galectin-3 stabilizes heterogeneous nuclear ribonucleoprotein Q to maintain proliferation of human colon cancer cells.  |  Yoo, BC., et al. 2009. Cell Mol Life Sci. 66: 350-64. PMID: 19137262
10. Heterogeneous nuclear ribonucleoprotein Q is a novel substrate of SH2 domain-containing phosphatase-2.  |  Watanabe, N., et al. 2013. J Biochem. 154: 475-80. PMID: 23946508
11. Oculopharyngeal muscular dystrophy mutations link the RNA-binding protein HNRNPQ to autophagosome biogenesis.  |  Ishtayeh, H., et al. 2023. Aging Cell. 22: e13949. PMID: 37559347
12. hnRNP H is a component of a splicing enhancer complex that activates a c-src alternative exon in neuronal cells.  |  Chou, MY., et al. 1999. Mol Cell Biol. 19: 69-77. PMID: 9858532

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-hnRNP Q Antibody (I8E4) has 14 citations in a variety of scientific publications.

Primary antibodies like Anti-hnRNP Q Antibody (I8E4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

hnRNP Q Antibody (I8E4) Specifications

Alternate Product Name: hnRNP Q

Product Clone Name: I8E4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: hnRNP-Q

Epitope Species: human

Product Reactivity/Detection: hnRNP Q

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: Xenopus

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 14 citations in various scientific publications

Available Conjugates/Forms for hnRNP Q Antibody (I8E4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin