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hnRNP M1-4 Antibody (3C181) - Western Blotting - Image 373413
hnRNP M1-4 Antibody (3C181): sc-56702. Western blot analysis of hnRNP M1-4 expression in Jurkat whole cell lysate.
hnRNP M1-4 Antibody (3C181): sc-56702. Western blot analysis of hnRNP M1-4 expression in Jurkat whole cell lysate.

hnRNP M1-4 Antibody (3C181): sc-56702

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Datasheets
  • hnRNP M1-4 Antibody (3C181) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
  • raised against full length native hnRNP M1-4 of human origin
  • recommended for detection of hnRNP M1-4 of mouse, rat, human, bovine and porcine origin by WB, IP and ELISA
  • See hnRNP M1-4 (1D8): sc-20002 for hnRNP M1-4 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for hnRNP M1-4 Antibody (3C181) for WB applications. These reagents are now offered in bundles with hnRNP M1-4 Antibody (3C181) (see ordering information below).
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Additional hnRNP Antibodies including hnRNP A, hnRNP A1, hnRNP A2, hnRNP B, hnRNP B1, hnRNP C1, hnRNP C2, hnRNP E1, hnRNP E2, hnRNP F, hnRNP H, hnRNP H3, hnRNP I, hnRNP J, hnRNP K and hnRNP L

hnRNP M1-4 Antibody (3C181) is a mouse monoclonal IgG1 antibody that detects hnRNP M in mouse, rat, human, bovine, and porcine samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). hnRNP M is a crucial component of the heterogeneous nuclear ribonucleoprotein family, playing a significant role in pre-mRNA splicing and the regulation of gene expression. hnRNP M is primarily located in the nucleus, where hnRNP M participates in the assembly of spliceosomal complexes, which are essential for the accurate processing of pre-mRNA into mature mRNA. Proper functioning of hnRNP M is vital for maintaining cellular homeostasis, as hnRNP M ensures that splicing occurs correctly, thereby influencing the production of functional proteins. Additionally, hnRNP M has been implicated in stress-induced splicing arrest, highlighting hnRNP M′s importance in cellular responses to environmental changes. Anti-hnRNP M antibody (3C181) is an invaluable tool for researchers studying mRNA processing and the intricate regulatory mechanisms that govern gene expression across various species.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

hnRNP M1-4 Antibody (3C181) References:

  1. The 72/74-kDa polypeptides of the 70-110 S large heterogeneous nuclear ribonucleoprotein complex (LH-nRNP) represent a discrete subset of the hnRNP M protein family.  |  Kafasla, P., et al. 2000. Biochem J. 350 Pt 2: 495-503. PMID: 10947964
  2. Turning on a fuel switch of cancer: hnRNP proteins regulate alternative splicing of pyruvate kinase mRNA.  |  Chen, M., et al. 2010. Cancer Res. 70: 8977-80. PMID: 20978194
  3. Cellular RNA binding proteins NS1-BP and hnRNP K regulate influenza A virus RNA splicing.  |  Tsai, PL., et al. 2013. PLoS Pathog. 9: e1003460. PMID: 23825951
  4. Selective interactions of hnRNP M isoforms with the TET proteins TAF15 and TLS/FUS.  |  Marko, M., et al. 2014. Mol Biol Rep. 41: 2687-95. PMID: 24474660
  5. Cholinergic Regulation of hnRNPA2/B1 Translation by M1 Muscarinic Receptors.  |  Kolisnyk, B., et al. 2016. J Neurosci. 36: 6287-96. PMID: 27277805
  6. ESCO2 promotes lung adenocarcinoma progression by regulating hnRNPA1 acetylation.  |  Zhu, HE., et al. 2021. J Exp Clin Cancer Res. 40: 64. PMID: 33573689
  7. HPV18 oncoproteins driven expression of PKM2 reprograms HeLa cell metabolism to maintain aerobic glycolysis and viability.  |  Prakasam, G., et al. 2022. Virusdisease. 33: 223-235. PMID: 36277414
  8. Both RNA-binding domains in heterogenous nuclear ribonucleoprotein A1 contribute toward single-stranded-RNA binding.  |  Shamoo, Y., et al. 1994. Biochemistry. 33: 8272-81. PMID: 7518244
  9. The human hnRNP M proteins: identification of a methionine/arginine-rich repeat motif in ribonucleoproteins.  |  Datar, KV., et al. 1993. Nucleic Acids Res. 21: 439-46. PMID: 8441656
  10. Proteins C1 and C2 of heterogeneous nuclear ribonucleoprotein complexes bind RNA in a highly cooperative fashion: support for their contiguous deposition on pre-mRNA during transcription.  |  McAfee, JG., et al. 1996. Biochemistry. 35: 1212-22. PMID: 8573576

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

hnRNP M1-4 Antibody (3C181)

sc-56702
200 µg/ml
$322.00

hnRNP M1-4 Antibody (3C181): m-IgG Fc BP-HRP Bundle

sc-539037
200 µg Ab; 10 µg BP
$361.00

hnRNP M1-4 Antibody (3C181): m-IgG1 BP-HRP Bundle

sc-541220
200 µg Ab; 20 µg BP
$361.00

How much sample do you recommend loading on the gel for Western blot detection using hnRNP M1-4 (3C181): sc-56702?

Asked by: Cweed
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Strong band with some background for westernStrong band with some background for western blot analysis of hnRNP M1-4 expression in HEK293 whole cell lysate. - SCBT QC
Date published: 2015-01-27
Rated 3 out of 5 by from Worked with nonWorked with non-transfected 293, human hnRNP M transfected 293 and HeLa whole cell lysates by WB with some background. - SCBT QC
Date published: 2015-01-17
Rated 4 out of 5 by from Works fine with some background for westernWorks fine with some background for western blot analysis of hnRNP M1-4 expression in HeLa, MDA-MB-231 and ME-180 whole cell lysates and HeLa nuclear extract. - SCBT QC
Date published: 2013-04-10
  • y_2026, m_5, d_5, h_11
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hnRNP M1-4 Antibody (3C181) is rated 3.7 out of 5 by 3.
  • y_2026, m_5, d_5, h_11
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.43
  • cp_1, bvpage1
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