hnRNP M1-4 Antibody (3C181) recommended for detection of hnRNP M1-4 of mouse, rat, human, bovine and porcine origin by WB, IP and ELISA

hnRNP M1-4 Antibody (3C181): sc-56702

hnRNP M1-4 Antibody (3C181) is rated 3.7 out of 5 by 3.
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Datasheets

    • hnRNP M1-4 Antibody (3C181) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against full length native hnRNP M1-4 of human origin
    • recommended for detection of hnRNP M1-4 of mouse, rat, human, bovine and porcine origin by WB, IP and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See hnRNP M1-4 (1D8): sc-20002 for hnRNP M1-4 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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How much sample do you recommend loading on the gel for Western blot detection using hnRNP M1-4 (3C181): sc-56702?

Asked by: Cweed
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Strong band with some background for western Strong band with some background for western blot analysis of hnRNP M1-4 expression in HEK293 whole cell lysate. - SCBT QC
Date published: 2015-01-27
Rated 3 out of 5 by from Worked with non Worked with non-transfected 293, human hnRNP M transfected 293 and HeLa whole cell lysates by WB with some background. - SCBT QC
Date published: 2015-01-17
Rated 4 out of 5 by from Works fine with some background for western Works fine with some background for western blot analysis of hnRNP M1-4 expression in HeLa, MDA-MB-231 and ME-180 whole cell lysates and HeLa nuclear extract. - SCBT QC
Date published: 2013-04-10
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