hnRNP M Antibody (5-RE36) recommended for detection of hnRNP M of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

hnRNP M Antibody (5-RE36): sc-134360

hnRNP M Antibody (5-RE36) is rated 4.3 out of 5 by 3.
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Datasheets

    • hnRNP M Antibody (5-RE36) is a mouse monoclonal IgG2a (kappa light chain) provided at 100 µg/ml
    • raised against recombinant hnRNP M protein of human origin
    • recommended for detection of hnRNP M of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See hnRNP M1-4 (1D8): sc-20002 for hnRNP M antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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What is the epitope sequence to which the antibody binds? I want to express truncated product

Asked by: sfu11
Thank you for your question. The epitope for this antibody maps within amino acids 50-120 of hnRNP M of human origin (accession#P52272). The exact epitope has not been mapped.
Answered by: Tech Service
Date published: 2019-08-14

Can sc-134360: hnRNP M (5-RE36) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Randy McDonald
Thank you for your question. Yes, sc-134360: hnRNP M (5-RE36) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Excellent! This antibody works great in western blot analysis of hnRNP M expression in Hep G2 whole cell lysate.
Date published: 2017-02-01
Rated 4 out of 5 by from Wonderful immunoperoxidase nuclear staining Wonderful immunoperoxidase nuclear staining in formalin-fixed, paraffin-embedded human thyroid nodular goiter tissue. -SCBT QC
Date published: 2015-05-06
Rated 4 out of 5 by from Good specificity and signal strength in Hep Good specificity and signal strength in Hep G2 whole cell lysate. -SCBT QC
Date published: 2015-03-12
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