hnRNP K Antibody (D-6): sc-28380

hnRNP K Antibody (D-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects hnRNP K of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-hnRNP K antibody (D-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The heterogeneous nuclear ribonucleoprotein K (hnRNP K) plays a crucial role in regulating gene expression, particularly in mRNA processing and transport. hnRNP K is primarily located in the nucleus, where binding to heterogeneous nuclear RNA (hnRNA) produced by RNA Polymerase II facilitates splicing and maturation of pre-mRNA. hnRNP K shuttles between the nucleus and cytoplasm, essential for function in both transcription and translation processes. Two K homologous (KH) domains mediate interactions with various molecular partners, enhancing post-transcriptional regulation. Post-translational modifications, including phosphorylation at specific sites such as Ser 302, are critical for modulating activity and interactions, influencing cellular functions such as DNA binding, RNA binding, and nucleocytoplasmic transport. This multifaceted functionality underscores hnRNP K′s importance in maintaining cellular homeostasis and responding to stress signals, making hnRNP K a significant target for research in gene regulation and related diseases.

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hnRNP K Antibody (D-6) References:

1. Regulated interaction of protein kinase Cdelta with the heterogeneous nuclear ribonucleoprotein K protein.  |  Schullery, DS., et al. 1999. J Biol Chem. 274: 15101-9. PMID: 10329716
2. Nuclear pre-mRNA compartmentalization: trafficking of released transcripts to splicing factor reservoirs.  |  Melcák, I., et al. 2000. Mol Biol Cell. 11: 497-510. PMID: 10679009
3. Protein-protein interaction among hnRNPs shuttling between nucleus and cytoplasm.  |  Kim, JH., et al. 2000. J Mol Biol. 298: 395-405. PMID: 10772858
4. Growth factors regulate heterogeneous nuclear ribonucleoprotein K expression and function.  |  Mandal, M., et al. 2001. J Biol Chem. 276: 9699-704. PMID: 11121407
5. LCDR regulates the integrity of lysosomal membrane by hnRNP K-stabilized LAPTM5 transcript and promotes cell survival.  |  Yang, X., et al. 2022. Proc Natl Acad Sci U S A. 119: PMID: 35091468
6. The cancer-testis lncRNA lnc-CTHCC promotes hepatocellular carcinogenesis by binding hnRNP K and activating YAP1 transcription.  |  Xia, A., et al. 2022. Nat Cancer. 3: 203-218. PMID: 35122073
7. HnRNP K regulates inflammatory gene expression by mediating splicing pattern of transcriptional factors.  |  Liu, S., et al. 2023. Exp Biol Med (Maywood). 248: 1479-1491. PMID: 35866661
8. hnRNP K Degrades Viral Nucleocapsid Protein and Induces Type I IFN Production to Inhibit Porcine Epidemic Diarrhea Virus Replication.  |  Qin, W., et al. 2022. J Virol. 96: e0155522. PMID: 36317879
9. hnRNP K induces HPV16 oncogene expression and promotes cervical cancerization.  |  Lyu, Y., et al. 2023. J Cancer Res Clin Oncol. 149: 6225-6237. PMID: 36700980
10. A nuclear localization domain in the hnRNP A1 protein.  |  Siomi, H. and Dreyfuss, G. 1995. J Cell Biol. 129: 551-60. PMID: 7730395
11. The pre-mRNA binding K protein contains a novel evolutionarily conserved motif.  |  Siomi, H., et al. 1993. Nucleic Acids Res. 21: 1193-8. PMID: 8464704
12. Identification and characterisation of a novel human RNA-binding protein.  |  Badolato, J., et al. 1995. Gene. 166: 323-7. PMID: 8543184

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-hnRNP K Antibody (D-6) has 91 citations in a variety of scientific publications.

Primary antibodies like Anti-hnRNP K Antibody (D-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

hnRNP K Antibody (D-6) Specifications

Alternate Product Name: hnRNP K

Product Clone Name: D-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-300

Epitope Target: hnRNP K

Epitope Species: human

Product Reactivity/Detection: hnRNP K

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 91 citations in various scientific publications

Available Conjugates/Forms for hnRNP K Antibody (D-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin