hnRNP H3 Double Nickase Plasmid (h): sc-404805-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- hnRNP H3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- hnRNP H3 Double Nickase Plasmid (h) and hnRNP H3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HNRNPH3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: hnRNP H3 Antibody (D-4): sc-376416
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
hnRNP H3 Double Nickase Plasmid (h) | sc-404805-NIC | 20 µg | $410.00 | |||
hnRNP H3 Double Nickase Plasmid (h2) | sc-404805-NIC-2 | 20 µg | $410.00 |
HNRNPH3 encodes the human heterogeneous nuclear ribonucleoprotein hnRNP H3, an RNA-binding protein that recognizes G-rich motifs and helps coordinate pre-mRNA splicing decisions. hnRNP H3 participates in spliceosome-associated regulation of alternative exon usage, coupling RNA processing with transcription and mRNA maturation. Through these functions it contributes to control of transcript isoform balance that shapes pathways such as cell-cycle regulation, stress responses, and RNA metabolism. Dysregulated hnRNP family activity and splicing-network perturbation are frequently studied in the context of oncogenic transformation and neurodegenerative disease mechanisms, making HNRNPH3 a useful target for interrogating splicing-dependent phenotypes.
hnRNP H3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HNRNPH3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HNRNPH3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HNRNPH3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HNRNPH3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.