Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
hnRNP E2 CRISPR/Cas9 KO Plasmid (h): sc-402274
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- hnRNP E2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the hnRNP E2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: hnRNP E2 Antibody (B-7): sc-514787
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
hnRNP E2 CRISPR/Cas9 KO Plasmid (h) | sc-402274 | 20 µg | $397.00 |
Overview
PCBP2 encodes the human RNA-binding protein hnRNP E2, a poly(C)-binding factor that associates with single-stranded RNA to regulate mRNA splicing, stability, nuclear export, and translation. hnRNP E2 participates in post-transcriptional gene control by coordinating ribonucleoprotein complexes that shape transcript fate and protein output, including modulation of UTR-dependent translational silencing and RNA processing programs. Through these activities, PCBP2 influences cellular stress responses, proliferation, and differentiation pathways, and is frequently studied in the context of dysregulated RNA metabolism. Altered PCBP2/hnRNP E2 function or expression has been linked to disease-relevant phenotypes, including oncogenic signaling and changes in host–virus interactions mediated by RNA regulatory networks.
hnRNP E2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PCBP2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PCBP2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PCBP2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish hnRNP E2 protein expression.
This CRISPR knockout system enables efficient generation of PCBP2-deficient cell models for investigation of hnRNP E2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PCBP2 exon(s) critical for hnRNP E2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PCBP2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by hnRNP E2 CRISPR/Cas9 KO Plasmid (h) and hnRNP E2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PCBP2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by hnRNP E2 HDR Plasmid (h) and hnRNP E2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PCBP2 homology arms to support homology-directed repair at defined PCBP2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.