Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
hnRNP E1 Double Nickase Plasmid (m): sc-423930-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- hnRNP E1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- hnRNP E1 Double Nickase Plasmid (m) and hnRNP E1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Pcbp1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: hnRNP E1 Antibody (E-2): sc-137249
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
hnRNP E1 Double Nickase Plasmid (m) | sc-423930-NIC | 20 µg | $410.00 | |||
hnRNP E1 Double Nickase Plasmid (m2) | sc-423930-NIC-2 | 20 µg | $410.00 |
Mouse Pcbp1 encodes heterogeneous nuclear ribonucleoprotein E1 (hnRNP E1), an RNA-binding protein that recognizes poly(C) motifs to control mRNA stability, translation, and alternative processing. hnRNP E1 participates in post-transcriptional regulatory networks that shape cell-cycle progression, differentiation programs, and stress-responsive gene expression, and it can couple RNA regulation to signal-dependent remodeling of gene output. Through its roles in ribonucleoprotein complex assembly and translational control, hnRNP E1 influences pathways linked to epithelial plasticity and cytoskeletal dynamics. Altered PCBP1/hnRNP E1 activity has been associated with dysregulated RNA metabolism in cancer-related contexts and other disorders where RNA processing fidelity is critical.
hnRNP E1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Pcbp1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Pcbp1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Pcbp1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Pcbp1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.