hnRNP C1/C2 CRISPR/Cas9 KO Plasmid (m): sc-420894

Overview

Hnrnpc encodes the heterogeneous nuclear ribonucleoprotein C1/C2 (hnRNP C1/C2), abundant nuclear RNA-binding proteins that assemble on pre-mRNAs to package nascent transcripts and shape RNA fate. hnRNP C participates in co-transcriptional splice-site choice, mRNA 3′ end processing, and nuclear retention/export decisions, and it helps maintain transcriptome integrity by antagonizing inappropriate Alu exonization and other cryptic splicing events. Through these functions, Hnrnpc integrates with core RNA metabolism pathways and influences gene expression programs linked to proliferation, differentiation, and cellular stress responses. Dysregulation of hnRNP C has been associated in the literature with altered splicing landscapes observed in cancer and neurodegeneration-relevant models, making it a common node for studying RNA-processing defects.

hnRNP C1/C2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Hnrnpc gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Hnrnpc together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Hnrnpc open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish hnRNP C1/C2 protein expression.

This CRISPR knockout system enables efficient generation of Hnrnpc-deficient cell models for investigation of hnRNP C1/C2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting Hnrnpc exon(s) critical for hnRNP C1/C2 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple Hnrnpc genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by hnRNP C1/C2 CRISPR/Cas9 KO Plasmid (m) and hnRNP C1/C2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Hnrnpc locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by hnRNP C1/C2 HDR Plasmid (m) and hnRNP C1/C2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Hnrnpc homology arms to support homology-directed repair at defined Hnrnpc target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.