hnRNP A2/B1 Antibody (EF-67): sc-53531

hnRNP A2/B1 Antibody (EF-67) is a mouse monoclonal IgG1 kappa light chain antibody that detects hnRNP A2/B1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Anti-hnRNP A2/B1 antibody (EF-67) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The heterogeneous nuclear ribonucleoprotein A2/B1 plays a crucial role in mRNA metabolism, particularly in pre-mRNA processing and splicing regulation. hnRNP A2/B1 is primarily located in the nucleus, where interactions with pre-mRNA and splicing factors influence splice site selection, ensuring proper maturation of mRNA transcripts. Nuclear-cytoplasmic shuttling of hnRNP A2/B1 allows dynamic regulation of gene expression in response to cellular signals. The A/B subfamily of hnRNPs, including hnRNP A2/B1, is essential for spliceosomal complex formation, making these proteins vital for accurate pre-mRNA processing. Understanding hnRNP A2/B1 function and localization helps elucidate gene regulation mechanisms and potential implications in cancer and neurodegenerative disorders.

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hnRNP A2/B1 Antibody (EF-67) References:

1. Nuclear pre-mRNA compartmentalization: trafficking of released transcripts to splicing factor reservoirs.  |  Melcák, I., et al. 2000. Mol Biol Cell. 11: 497-510. PMID: 10679009
2. Protein-protein interaction among hnRNPs shuttling between nucleus and cytoplasm.  |  Kim, JH., et al. 2000. J Mol Biol. 298: 395-405. PMID: 10772858
3. hnRNP A2/B1 binds specifically to single stranded vertebrate telomeric repeat TTAGGGn.  |  McKay, SJ. and Cooke, H. 1992. Nucleic Acids Res. 20: 6461-4. PMID: 1282701
4. Expression of hnRNP A2/B1 proteins in small airway epithelial cells.  |  Satoh, H., et al. 2004. Int J Mol Med. 14: 605-8. PMID: 15375589
5. hnRNP A2/B1 modulates epithelial-mesenchymal transition in lung cancer cell lines.  |  Tauler, J., et al. 2010. Cancer Res. 70: 7137-47. PMID: 20807810
6. hnRNP A2/B1 interacts with influenza A viral protein NS1 and inhibits virus replication potentially through suppressing NS1 RNA/protein levels and NS1 mRNA nuclear export.  |  Wang, Y., et al. 2014. Virology. 449: 53-61. PMID: 24418537
7. Cilostazol inhibits the expression of hnRNP A2/B1 and cytokines in human dermal microvascular endothelial cells.  |  An, Y., et al. 2017. Clin Exp Rheumatol. 35 Suppl 108: 60-66. PMID: 28850024
8. Isolation and characterization of a novel, low abundance hnRNP protein: A0.  |  Myer, VE. and Steitz, JA. 1995. RNA. 1: 171-82. PMID: 7585247
9. A nuclear localization domain in the hnRNP A1 protein.  |  Siomi, H. and Dreyfuss, G. 1995. J Cell Biol. 129: 551-60. PMID: 7730395
10. Three new members of the RNP protein family in Xenopus.  |  Good, PJ., et al. 1993. Nucleic Acids Res. 21: 999-1006. PMID: 8451200
11. Identification and characterisation of a novel human RNA-binding protein.  |  Badolato, J., et al. 1995. Gene. 166: 323-7. PMID: 8543184
12. Regulated tissue-specific expression of antagonistic pre-mRNA splicing factors.  |  Hanamura, A., et al. 1998. RNA. 4: 430-44. PMID: 9630249

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-hnRNP A2/B1 Antibody (EF-67) has 38 citations in a variety of scientific publications.

Primary antibodies like Anti-hnRNP A2/B1 Antibody (EF-67) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

hnRNP A2/B1 Antibody (EF-67) Specifications

Alternate Product Name: hnRNP A2/B1

Product Clone Name: EF-67

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: hnRNP A2

Epitope Species: human

Product Reactivity/Detection: hnRNP A2/B1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 38 citations in various scientific publications

Available Conjugates/Forms for hnRNP A2/B1 Antibody (EF-67): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin