HNF-6 CRISPR/Cas9 KO Plasmid (m): sc-420893
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HNF-6 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HNF-6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HNF-6 Antibody (G-10): sc-376167
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HNF-6 CRISPR/Cas9 KO Plasmid (m) | sc-420893 | 20 µg | $397.00 |
Overview
Onecut1 encodes the homeobox transcription factor HNF-6, a key regulator of endoderm-derived tissue differentiation in mouse, including pancreatic, hepatic, and biliary lineages. HNF-6 binds cis-regulatory elements to coordinate transcriptional programs controlling epithelial morphogenesis, ductal development, and cell fate decisions, and it functionally intersects with Notch, Wnt/β-catenin, and TGF-β signaling during organogenesis and tissue homeostasis. Altered Onecut1 activity has been linked to defects in pancreatic endocrine development and ductal patterning, making it relevant to studies of metabolic dysfunction and developmental phenotypes. In experimental systems, Onecut1/HNF-6 perturbation is used to dissect transcriptional networks that couple chromatin state to lineage specification and organ maturation.
HNF-6 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Onecut1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Onecut1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Onecut1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HNF-6 protein expression.
This CRISPR knockout system enables efficient generation of Onecut1-deficient cell models for investigation of HNF-6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Onecut1 exon(s) critical for HNF-6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Onecut1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HNF-6 CRISPR/Cas9 KO Plasmid (m) and HNF-6 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Onecut1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HNF-6 HDR Plasmid (m) and HNF-6 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Onecut1 homology arms to support homology-directed repair at defined Onecut1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.