HMG-1/HMGB1 (m): 293T Lysate: sc-120823

The HMG-1 (m): 293T Lysate is derived from the HEK293T cell line, a human embryonic kidney cell line known for its high transfection efficiency and widespread use in molecular biology research. This lysate contains High Mobility Group Box 1 (HMG-1), also known as HMGB1, a highly conserved non-histone chromosomal protein that plays a pivotal role in DNA architecture and gene regulation. HMG-1 functions by binding to DNA, bending it and facilitating the assembly of nucleoprotein complexes that are crucial for processes such as transcription, replication, and DNA repair. It also acts as a damage-associated molecular pattern (DAMP) molecule when released extracellularly, signaling through receptors such as RAGE and TLR4 to modulate inflammatory responses. In research, the HMG-1 (m): 293T Lysate is utilized to investigate the multifaceted roles of HMG-1 in both nuclear and extracellular environments. Researchers use this lysate in in vitro assays to study HMG-1's interactions with DNA and other nuclear proteins, analyze its role in chromatin remodeling, and explore the regulatory mechanisms governing its intracellular and extracellular functions. The lysate is particularly valuable for examining the signaling pathways activated by extracellular HMG-1 and its impact on immune cell behavior. Additionally, it aids in screening for modulators of HMG-1 activity, providing insights into how this protein influences cellular processes and contributes to inflammatory responses.