Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HMG-1/HMGB1 Double Nickase Plasmid (h): sc-400735-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HMG-1/HMGB1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HMG-1/HMGB1 Double Nickase Plasmid (h) and HMG-1/HMGB1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HMGB1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HMG-1/HMGB1 Antibody (D-3): sc-518191
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HMG-1/HMGB1 Double Nickase Plasmid (h) | sc-400735-NIC | 20 µg | $410.00 | |||
HMG-1/HMGB1 Double Nickase Plasmid (h2) | sc-400735-NIC-2 | 20 µg | $410.00 |
HMGB1 (HMG-1) encodes a highly conserved chromatin-associated DNA-binding protein that bends DNA and organizes nucleoprotein complexes, influencing transcription, replication, recombination, and DNA repair. In the nucleus, HMGB1 participates in genome stability pathways including base excision repair and double-strand break responses, while its extracellular release can function as a damage-associated molecular pattern that modulates innate immune signaling. Through interactions with receptors such as RAGE and TLRs, HMGB1 links cellular stress to inflammatory programs that shape tumor microenvironments and tissue injury responses. Dysregulated HMGB1 activity and localization have been associated with cancer biology, autoimmunity, and inflammatory pathology, supporting its use as a mechanistic node in studies of chromatin dynamics and immunometabolism.
HMG-1/HMGB1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HMGB1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HMGB1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HMGB1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HMGB1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.