HM74 Antibody (D-8): sc-377292

HM74 Antibody (D-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects HM74 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). HM74 (D-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HM74, also known as PUMAG or Puma-g, is a member of the G protein-coupled receptor (GPCR) superfamily, which plays a crucial role in cellular signaling. Specifically, HM74 is a G(i) protein-coupled receptor that mediates nicotinic acid′s metabolic effects, making HM74 significant in regulating lipid metabolism and energy homeostasis. This receptor is predominantly found in the cell membrane and is highly expressed in adipocytes, immune cells, and the spleen, highlighting HM74′s importance in metabolic processes and immune responses. Notably, HM74 is encoded by two different genes in humans (GPR109A and GPR109B), resulting in two homologous proteins, HM74A and HM74B, which share 96% sequence similarity. In contrast, mice and rats possess a single gene (Gpr109a) that encodes HM74 . HM74′s unique structural features, including seven transmembrane domains and the absence of N-linked glycosylation sites near the N-terminus, suggest distinct ligand specificity compared to other GPCR family members, further emphasizing HM74′s specialized role in metabolic regulation.

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HM74 Antibody (D-8) References:

1. Molecular identification of high and low affinity receptors for nicotinic acid.  |  Wise, A., et al. 2003. J Biol Chem. 278: 9869-74. PMID: 12522134
2. PUMA-G and HM74 are receptors for nicotinic acid and mediate its anti-lipolytic effect.  |  Tunaru, S., et al. 2003. Nat Med. 9: 352-5. PMID: 12563315
3. Molecular identification of nicotinic acid receptor.  |  Soga, T., et al. 2003. Biochem Biophys Res Commun. 303: 364-9. PMID: 12646212
4. Niacin therapy in atherosclerosis.  |  Meyers, CD., et al. 2004. Curr Opin Lipidol. 15: 659-65. PMID: 15529025
5. Variations in human HM74 (GPR109B) and HM74A (GPR109A) niacin receptors.  |  Zellner, C., et al. 2005. Hum Mutat. 25: 18-21. PMID: 15580557
6. Niacin mediates lipolysis in adipose tissue through its G-protein coupled receptor HM74A.  |  Zhang, Y., et al. 2005. Biochem Biophys Res Commun. 334: 729-32. PMID: 16018973
7. Flushing out the role of GPR109A (HM74A) in the clinical efficacy of nicotinic acid.  |  Pike, NB. 2005. J Clin Invest. 115: 3400-3. PMID: 16322787
8. Niacin induces PPARgamma expression and transcriptional activation in macrophages via HM74 and HM74a-mediated induction of prostaglandin synthesis pathways.  |  Knowles, HJ., et al. 2006. Biochem Pharmacol. 71: 646-56. PMID: 16386710
9. Nicotinic acid receptor subtypes and their ligands.  |  Soudijn, W., et al. 2007. Med Res Rev. 27: 417-33. PMID: 17238156
10. Neutrophil apoptosis mediated by nicotinic acid receptors (GPR109A).  |  Kostylina, G., et al. 2008. Cell Death Differ. 15: 134-42. PMID: 17932499
11. Nicotinic acid (niacin) receptor agonists: will they be useful therapeutic agents?  |  Kamanna, VS. and Kashyap, ML. 2007. Am J Cardiol. 100: S53-61. PMID: 18047854
12. Molecular cloning of cDNAs encoding a LD78 receptor and putative leukocyte chemotactic peptide receptors.  |  Nomura, H., et al. 1993. Int Immunol. 5: 1239-49. PMID: 7505609

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HM74 Antibody (D-8) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-HM74 Antibody (D-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HM74 Antibody (D-8) Specifications

Alternate Product Name: HM74

Product Clone Name: D-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 300-363

Epitope Target: HM74A

Epitope Species: human

Product Reactivity/Detection: HM74A and HM74B of human origin, and HM74 of mouse and rat origin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for HM74 Antibody (D-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin