HLA-G (h): 293T Lysate: sc-159408
- human HLA-G transfected 293T lysate
- 100 µg protein in 200 µl SDS-PAGE buffer; suitable as Western Blotting control for HLA-G antibodies
- Suitable control antibody: HLA-G Antibody (MEM-G/1): sc-51674 is recommended as control antibody for monitoring of HLA-G expression knockdown by Western blotting or immunofluorescence
- should be stored at -20° C and repeated freezing and thawing should be minimized
- sample vial should be boiled once prior to use
- non-transfected control 293T lysate (sc-117752)
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The HLA-G (h): 293T Lysate is derived from the HEK293T cell line, a human embryonic kidney cell line known for its high transfection efficiency and utility in a variety of research applications. This lysate contains Human Leukocyte Antigen G (HLA-G), a non-classical major histocompatibility complex (MHC) class I molecule that plays a significant role in immune tolerance and modulation. HLA-G is uniquely expressed in specific tissues such as the placenta, where it helps to prevent the maternal immune system from attacking the fetus. Mechanistically, HLA-G binds to inhibitory receptors like ILT2, ILT4, and KIR2DL4 on immune cells, leading to the suppression of natural killer (NK) cell and cytotoxic T lymphocyte (CTL) activity, as well as the induction of regulatory T cells (Tregs). In research, the HLA-G (h): 293T Lysate is extensively used to explore the mechanisms of immune modulation and tolerance. Researchers utilize this lysate in in vitro assays to study the interaction between HLA-G and its receptors, investigate the signaling pathways involved in immune suppression, and examine the effects of HLA-G on immune cell function. This lysate is particularly valuable for analyzing how HLA-G contributes to immune evasion and regulation, as well as its role in various physiological and pathological contexts.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HLA-G (h): 293T Lysate | sc-159408 | 100 µg/200 µl | $236.00 |