HLA-DRβ Antibody (DA2): sc-53314
- HLA-DRβ Antibody (DA2) is a mouse monoclonal IgG1 κ HLA-DRβ antibody, cited in 3 publications, provided at 200 µg/ml
- raised against the membranes of LKT cells of human origin
- HLA-DR beta Antibody (DA2) is recommended for detection of HLA-DRβ of human origin by WB, IP, IF and FCM
- Anti-HLA-DR beta Antibody (DA2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for HLA-DRβ Antibody (DA2) for WB applications. These reagents are now offered in bundles with HLA-DRβ Antibody (DA2) (see ordering information below).
HLA-DRβ Antibody (DA2) is a mouse monoclonal IgG1 kappa light chain antibody that detects the HLA-DR beta protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). HLA-DRβ (DA2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HLA-DR beta protein plays a crucial role in the immune response by forming part of the major histocompatibility complex (MHC) class II molecules, which are essential for presenting processed antigens to CD4+ T helper cells. This interaction is vital for the activation of the adaptive immune response, as CD4+ T cells recognize and respond to pathogens effectively. HLA-DR beta protein is primarily located on the surface of antigen-presenting cells, such as B cells, dendritic cells, and macrophages, where HLA-DR beta protein facilitates the presentation of peptide antigens derived from extracellular proteins. Proper functioning of HLA-DR beta protein is critical for immune surveillance and the maintenance of immune tolerance, and any dysfunction in this process can lead to autoimmune diseases or impaired immune responses. Additionally, polymorphisms in the HLA-DR gene have been associated with various autoimmune conditions, highlighting HLA-DR beta protein′s importance in both health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HLA-DRβ Antibody (DA2) References:
- HLA-DMA and HLA-DMB alleles in German patients with type 1 diabetes mellitus. | Siegmund, T., et al. 1999. Tissue Antigens. 54: 291-4. PMID: 10519369
- The association of HLA-DM genes with rheumatoid arthritis in Eastern France. | Toussirot, E., et al. 2000. Hum Immunol. 61: 303-8. PMID: 10689120
- Functional HLA-DM on the surface of B cells and immature dendritic cells. | Arndt, SO., et al. 2000. EMBO J. 19: 1241-51. PMID: 10716924
- Functional characterization of a lysosomal sorting motif in the cytoplasmic tail of HLA-DObeta. | Brunet, A., et al. 2000. J Biol Chem. 275: 37062-71. PMID: 10964920
- The down-regulation of HLA-DM gene expression in rheumatoid arthritis is not related to their promoter polymorphism. | Louis-Plence, P., et al. 2000. J Immunol. 165: 4861-9. PMID: 11046010
- Determination of the HLA-DM interaction site on HLA-DR molecules. | Doebele, RC., et al. 2000. Immunity. 13: 517-27. PMID: 11070170
- Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen. | Horejsí, V., et al. 1986. Folia Biol (Praha). 32: 12-25. PMID: 2422063
- Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens. | Horejsí, V., et al. 1986. Tissue Antigens. 28: 288-97. PMID: 2435017
- A role for HLA-DO as a co-chaperone of HLA-DM in peptide loading of MHC class II molecules. | Kropshofer, H., et al. 1998. EMBO J. 17: 2971-81. PMID: 9606180
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HLA-DRβ Antibody (DA2) | sc-53314 | 200 µg/ml | $322.00 | |||
HLA-DRβ Antibody (DA2): m-IgG Fc BP-HRP Bundle | sc-526794 | 200 µg Ab; 10 µg BP | $361.00 | |||
HLA-DRβ Antibody (DA2): m-IgG1 BP-HRP Bundle | sc-532167 | 200 µg Ab; 20 µg BP | $361.00 | |||
HLA-DRβ Antibody (DA2) AC | sc-53314 AC | 500 µg/ml, 25% agarose | $424.00 | |||
HLA-DRβ Antibody (DA2) HRP | sc-53314 HRP | 200 µg/ml | $322.00 | |||
HLA-DRβ Antibody (DA2) FITC | sc-53314 FITC | 200 µg/ml | $336.00 | |||
HLA-DRβ Antibody (DA2) PE | sc-53314 PE | 200 µg/ml | $349.00 | |||
HLA-DRβ Antibody (DA2) Alexa Fluor® 488 | sc-53314 AF488 | 200 µg/ml | $364.00 | |||
HLA-DRβ Antibody (DA2) Alexa Fluor® 546 | sc-53314 AF546 | 200 µg/ml | $364.00 | |||
HLA-DRβ Antibody (DA2) Alexa Fluor® 594 | sc-53314 AF594 | 200 µg/ml | $364.00 | |||
HLA-DRβ Antibody (DA2) Alexa Fluor® 647 | sc-53314 AF647 | 200 µg/ml | $364.00 | |||
HLA-DRβ Antibody (DA2) Alexa Fluor® 680 | sc-53314 AF680 | 200 µg/ml | $364.00 | |||
HLA-DRβ Antibody (DA2) Alexa Fluor® 790 | sc-53314 AF790 | 200 µg/ml | $364.00 |